显微镜知识库

显微镜知识库

显微镜知识库

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记,你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区,分享您的专业知识!
Image of glass with primary corundum having numerous inclusions. It was taken with a microscope using polarization contrast.

如玻璃般清晰的质量——偏光显微镜在玻璃生产中的应用

精致美味的饮料应搭配高品质的玻璃杯。早在古罗马时期,人们便开始制作精美如艺术品的酒杯。在中世纪,威尼斯的玻璃制造商因其玻璃的纯净度而广为人知。作为已知的人类使用的最古老的材料之一,玻璃广泛应用于众多领域,而且无一例外均须满足最严格的质量标准。
OCSS Cataract Surgery Symposium

Expert Techniques for Superior Visualization in Cataract Surgery

Join renowned ophthalmic surgeons, Dr. Hussein Almuhtaseb and Mr. Simon Madge, as they share their clinical expertise and real-world surgical strategies during the 2025 Online Cataract Surgery…
LMD ionizer before & after cut.

Eliminating Electrostatic Interference in Laser Microdissection

Electrostatic charge in laser microdissection (LMD) causes two critical failures: samples stick to charged surfaces and are lost, or samples fly into adjacent wells and cause cross-contamination. We…

History, Developments and Trends of Microscopy in Cancer Research

Cancer is a global disease, with 18 million new cases diagnosed and 10 million cancer-related deaths worldwide in 2020. This burden is set to increase, with a projected increase in cases of ~55% by…
小鼠成纤维细胞,F-肌动蛋白用 FITC 标记(绿色),微管蛋白用 Cy5 标记(红色),细胞核用 DAPI 标记(蓝色)。图片由德国海德堡马克斯-普朗克医学研究所 Günter Giese 博士提供。

荧光染料应用和特性概述

本文将介绍常用的荧光染料并概述其特性。荧光显微镜借助荧光染料、荧光蛋白或使用抗体的免疫荧光染色来研究特定的细胞成分。由于荧光剂种类繁多,荧光显微镜可用于检查蛋白质、核酸、聚糖、细胞器和其他细胞结构。

Researchers Insights: Microscopy in Cancer Research

Discover how imaging techniques are driving cancer research forward. In this issue, we present comprehensive multimodal studies using microscopy, as well as new directions in intraoperative cancer…
Leica Microsystems Life Science Product

Predictive Service Prevents Downtime in Ghent

At the VIB BioImaging Core in Ghent, Belgium, researchers depend on Leica’s Stellaris 8 confocal microscope to explore the frontiers of biomedical science. When Leica’s RemoteCare system detected a…
Zebrafish heart, DAPI (nuclei, blue), Tropomyosin (cardiomyocytes, red) and GFP (primordial cardiac layer, green). Courtesy of Anna Jazwinska, University of Fribourg, Switzerland.

显微镜中的荧光

荧光显微技术是一种特殊的光学显微镜技术。它利用的是荧光色素在一定波长的光激发下发光的能力。通过抗体染色或荧光蛋白标记,可以用这种荧光色素标记感兴趣的蛋白质。这样就可以确定单分子物种的分布、数量及其在细胞内的定位。此外,还可以进行共定位和相互作用研究,使用可逆结合染料(如 Ca2+ 和 fura-2)观察离子浓度,以及观察细胞的内吞和外吞过程。如今,利用荧光显微镜甚至可以对亚分辨率颗粒进行成像。
Some 2D measurements, e.g., lengths and areas, made on a PCB sample with a Leica measurement microscope using the Enersight software.

如何选择合适的测量显微镜

使用测量显微镜,用户可以测量样品特征的二维和三维尺寸,这对检测、质量控制、故障分析和研发&D 至关重要。然而,选择合适的显微镜需要评估应用需求以及显微镜的性能、易用性和灵活性。 如今,测量通常以数字方式进行,即使用带有摄像头和软件的显微镜,图像显示在显示器上,而不是通过目镜网线,从而提高了精度和可重复性。使用合适的测量显微镜可靠、快速地分析样品。
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