What is digital light-sheet (DLS) imaging?
Light-sheet microscopy, also referred to as selective-plane illumination microscopy, is a gentle way of imaging sensitive samples or fast biological processes in live specimens. The optical scanning is only done in a single plane and detected from the perpendicular direction. The excitation is done with a thin light-sheet that illuminates the focal plane and spares the sample from out-of-focus excitation. DLS microscopy uses a digital camera and is advantageous for fast imaging of live specimens. For more details, refer to the article: Confocal and Digital Light Sheet Imaging