类器官和3D细胞培养
生命科学研究中最令人振奋的最新进展之一是3D细胞培养系统的发展，例如类器官、球状体或器官芯片模型。 3D细胞培养物是一种人工环境，在这种环境中，细胞能够在三维空间中生长并与周围环境相互作用。 这些环境条件与它们在体内的情况相似。 类器官是一种3D细胞培养物，包含器官特异性细胞类型，可以表现出器官的空间组织和复制器官的某些功能。 类器官重现了一个生理上高度相关的系统，使研究人员能够研究复杂的多维度问题，例如疾病发作、组织再生和器官之间的相互作用。 光学显微镜是用类器官研究复杂的相互作用与关系的重要方法。
徕卡成像解决方案支持这些多功能样本的研究，使用这些系统可进行深度快速成像，适合终点测量或者通过活细胞成像进行动力学研究。
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克服类器官成像中的挑战
成像是研究类器官和球状体等3D细胞培养物的关键技术。
类器官的有效成像构成一系列的新挑战，因为它们包含很大的体积。 类器官可以被固定、进行免疫标记，并使用透明技术进行研究，以便对其3D结构成像。 通常，这些研究使用共聚焦显微镜进行，因为对于宽场系统而言，对细胞层多于2-3个的培养物成像可能具有很大的挑战性，宽场系统固有的模糊现象会掩盖感兴趣的信号。
类器官也可以用来研究动态过程。 活体类器官研究会面临典型的成像问题，例如光毒性和低信噪比，特别是在样本深度成像时。 最近，各种快速采集显微成像方法（如 FLIM 或光片）在活体类器官研究中受到青睐，因为使用这些方法时可以不改变样本的生理机能。
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