显微镜知识库

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

3D rendering of a mouse beta cell with mitochondria (blue), insulin SGs (orange), microtubules (red), nucleus (yellow), and plasma membrane (transparent).

High-Pressure Freezing Protocols for Ultrastructural 3D EM

High pressure freezing (HPF) can help preserve hydrated cells and tissues close to their biological state at the moment of immobilization, supporting more reliable ultrastructural interpretation than…

Ultramicrotome UC Enuity in Practice: Stable 15 nm Sections at ZFE

After using the UCT and UC6 ultramicrotomes, Claudia Mayrhofer calls UC Enuity a leap in stability—so robust that vibrations and temperature shifts don’t spoil sections, even with multiple users. Auto…

利用偏光显微镜优势确保玻璃质量

玻璃是已知最古老的材料之一。如今，玻璃被广泛应用于各种领域，如光学仪器、门窗、太阳能电池板、食品、饮料和药品容器等，因此必须符合严格的玻璃质量标准，尤其是光学玻璃。利用偏光显微镜对平板玻璃、中空玻璃和压制玻璃进行质量控制既快捷又经济。无需进行耗时的样品制备，即可对结节、金属、晶体夹杂物和气泡等缺陷进行分析。

Expert Techniques for Superior Visualization in Cataract Surgery

Join renowned ophthalmic surgeons, Dr. Hussein Almuhtaseb and Mr. Simon Madge, as they share their clinical expertise and real-world surgical strategies during the 2025 Online Cataract Surgery…

消除激光显微切割中的静电干扰

激光显微切割（LMD）中的静电荷会导致两种严重问题：样品粘附在带电表面而丢失，或者样品飞入相邻的孔中造成交叉污染。我们在 Leica LMD7 环境室中集成了一个离子发生器除静电。离子发生器将静电位移从 16%（16/100 次切割）降低到 0.2%（1/450+ 次切割）。低吸附 384 孔板的收集率从 65-75% 提高到 85-95%。

History, Developments and Trends of Microscopy in Cancer Research

Cancer is a global disease, with 18 million new cases diagnosed and 10 million cancer-related deaths worldwide in 2020. This burden is set to increase, with a projected increase in cases of ~55% by…

小鼠成纤维细胞，F-肌动蛋白用 FITC 标记（绿色），微管蛋白用 Cy5 标记（红色），细胞核用 DAPI 标记（蓝色）。图片由德国海德堡马克斯-普朗克医学研究所 Günter Giese 博士提供。

荧光染料应用和特性概述

本文将介绍常用的荧光染料并概述其特性。荧光显微镜借助荧光染料、荧光蛋白或使用抗体的免疫荧光染色来研究特定的细胞成分。由于荧光剂种类繁多，荧光显微镜可用于检查蛋白质、核酸、聚糖、细胞器和其他细胞结构。

Researchers Insights: Microscopy in Cancer Research

Discover how imaging techniques are driving cancer research forward. In this issue, we present comprehensive multimodal studies using microscopy, as well as new directions in intraoperative cancer…

Predictive Service Prevents Downtime in Ghent

At the VIB BioImaging Core in Ghent, Belgium, researchers depend on Leica’s Stellaris 8 confocal microscope to explore the frontiers of biomedical science. When Leica’s RemoteCare system detected a…

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