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Fluorescence Recovery after Photobleaching (FRAP) and its Offspring
FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…
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共聚焦光学截面厚度
共聚焦显微镜用于光学切片比较厚的样本。最直接的问题是:1. 什么是“比较厚的样本”;2. 光学切片到底有多厚?这两个问题在一定程度上是相关的,即假设一份厚样本至少比光学切片厚10倍。