显微镜知识库

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

Snapshot from a time lapse of a calcium imaging experiment using the ratiometric calcium indicator Fura-2.

比例成像

细胞的许多基本功能在很大程度上依赖于离子（例如钙、镁）、电压势和细胞质与周围细胞外空间之间的 pH 值的微妙但动态的平衡。这些平衡的变化会显著改变细胞的行为和功能。因此，实时测量细胞内离子、电压和 pH…

Ribbon diagram of a donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).

荧光寿命成像与荧光共振能量转移

荧光寿命是荧光团在发射荧光光子返回基态之前保持其激发态的平均时间长度。这取决于荧光团的分子组成和纳米环境。 FLIM将寿命测量与成像相结合：对每个图像像素以测得的荧光寿命进行颜色编码，产生额外的图像反差。因此，FLIM可以提供关于荧光分子空间分布的信息和有关其生化状态或纳米环境的信息。…

Proveo 8x 3D digital microscope used in ophthalmic surgery, illustrating heads‑up visualization and clinical collaboration.

4 Key Benefits of 3D Digital Microscopy in Ophthalmic Surgery

3D digital visualization is rapidly transforming ophthalmic surgery. Modern 3D surgical microscopes enable surgeons to perform procedures using high-resolution digital displays rather than traditional…

3D rendering of a mouse beta cell with mitochondria (blue), insulin SGs (orange), microtubules (red), nucleus (yellow), and plasma membrane (transparent).

High-Pressure Freezing Protocols for Ultrastructural 3D EM

High pressure freezing (HPF) can help preserve hydrated cells and tissues close to their biological state at the moment of immobilization, supporting more reliable ultrastructural interpretation than…

Ultramicrotome UC Enuity in Practice: Stable 15 nm Sections at ZFE

After using the UCT and UC6 ultramicrotomes, Claudia Mayrhofer calls UC Enuity a leap in stability—so robust that vibrations and temperature shifts don’t spoil sections, even with multiple users. Auto…

利用偏光显微镜优势确保玻璃质量

玻璃是已知最古老的材料之一。如今，玻璃被广泛应用于各种领域，如光学仪器、门窗、太阳能电池板、食品、饮料和药品容器等，因此必须符合严格的玻璃质量标准，尤其是光学玻璃。利用偏光显微镜对平板玻璃、中空玻璃和压制玻璃进行质量控制既快捷又经济。无需进行耗时的样品制备，即可对结节、金属、晶体夹杂物和气泡等缺陷进行分析。

Expert Techniques for Superior Visualization in Cataract Surgery

Join renowned ophthalmic surgeons, Dr. Hussein Almuhtaseb and Mr. Simon Madge, as they share their clinical expertise and real-world surgical strategies during the 2025 Online Cataract Surgery…

消除激光显微切割中的静电干扰

激光显微切割（LMD）中的静电荷会导致两种严重问题：样品粘附在带电表面而丢失，或者样品飞入相邻的孔中造成交叉污染。我们在 Leica LMD7 环境室中集成了一个离子发生器除静电。离子发生器将静电位移从 16%（16/100 次切割）降低到 0.2%（1/450+ 次切割）。低吸附 384 孔板的收集率从 65-75% 提高到 85-95%。

History, Developments and Trends of Microscopy in Cancer Research

Cancer is a global disease, with 18 million new cases diagnosed and 10 million cancer-related deaths worldwide in 2020. This burden is set to increase, with a projected increase in cases of ~55% by…

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