显微镜知识库

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

Image of burrs (red arrows) at the edge of a battery electrode acquired with a DVM6 digital microscope.

电池制造过程中的毛刺检测

毛刺是电池电极片边缘可能出现的缺陷，例如在制造过程中的分切环节。它们可能会因诸如短路等故障导致电池性能下降，并引发安全和可靠性问题。毛刺检测是电池生产质量控制的重要部分，对于生产具有可靠性能和寿命的电池至关重要。通过适当照明的光学显微镜可以在生产过程的关键步骤中快速可靠地对电极上的毛刺进行视觉检测。

Ultramicrotomy eBook: Targeting, Trimming & Alignment

Ultramicrotomy is evolving rapidly, and today’s microscopes demand high‑quality sections, precise targeting, and reproducible workflows. This eBook brings together expert application notes, automated…

Surgical incision in minimally invasive spine surgery (MISS) case. Image courtesy of Dr. A. Alfieri, Chief Physician & Head of Clinic of Neurosurgery & Spinal Surgery, Cantonal Hospital Winterthur, Switzerland.

Flexibility and Efficiency in Minimally Invasive Spine Surgery

According to Prof. Alex Alfieri, Chief Physician and Head of clinic for Neurosurgery and Spinal surgery at the Cantonal Hospital Winterthur, Minimally invasive spine surgery (MISS) is transforming…

Foraminifera (Ammonia confertitesta) labeled with membrane-permeable calcein, high-pressure frozen in salt water using EM ICE. The sample was cryo-planed and targeted with the M205 on the Cryo-Fluo Enuity, then transferred under cryo conditions to the Cryo-Stellaris for widefield and confocal imaging, revealing details of the staining pattern. Image courtesy: David Evans, University of Southampton.

High-Pressure Freezing for Organoids: Cryo CLEM & FIB Lift Out

Master cryo EM workflow steps for challenging 3D samples: when to choose HPF vs. plunge freezing, reproducible blotting/ice control, contamination aware transfers, Cryo CLEM 3D targeting in organoids,…

Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imager with a 2x, 0.15 NA objective at 3.4x zoom was used to produce this 425 µm Z-stack (26 positions) which is presented here as an Extended Depth of Field (EDoF) projection. Images courtesy of Dr. F. Birey, Dr. S. Pasca laboratory, Palo Alto, CA.

Guide to Live-Cell Imaging

For a wide range of applications in various research fields of life science, live-cell imaging is an indispensable tool for visualizing cells in a state as close to in vivo, i.e. living and active, as…

神经外科和眼科中的融合光学 - 更大三维聚焦区域

神经外科医生和眼科医生处理精细结构、深或狭窄的腔体以及具有至关重要功能的微小结构。因此，手术区域的清晰三维视图对手术结果和患者安全至关重要。到目前为止，增加景深以获得更大三维聚焦区域只能通过降低分辨率来实现。一项新技术能够克服这一挑战。

Electron microscope (EM) image of a cross section of C. elegans (roundworm). Courtesy of T. Müller-Reichert, MPI-CBG, Dresden, Germany and K. McDonald, University of California, Berkeley, USA.

高压冷冻简介

水是细胞最主要的组成部分，因此对于维持细胞超微结构至关重要。目前，冷冻固定是固定细胞成分，而不导致其显著结构变化的唯一途径。现阶段有两种常见的方法：投入冷冻与高压冷冻固定。

Shown is the DMi8 inverted microscope which is used for life-science research.

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…

颌面整形与重建手术中的先进可视化技术

整形与重建手术要求极高。手术显微镜发挥着重要作用，能确保皮瓣血管化良好。 Dr. Christine Bach 是法国 Suresnes 地区 Foch 医院的整形与重建外科医生，专攻头颈部手术，为耳鼻喉癌症患者实施重建手术。

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