显微镜知识库

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

Dr. Andrew Huang, Baylor College of Medicine, in the operating room (OR) performing ear, nose and throat (ENT) surgery using the MyVeo surgical visualization headset.

A Microvascular Surgeon’s View: How MyVeo Transforms Visualization

In this article, Dr. Andrew T. Huang, MD, FACS, otolaryngologist and a head and neck reconstructive surgeon, shares how digital 3D surgical visualization with the MyVeo headset from Leica Microsystems…

Optic nerve regeneration in the Xenopus laevis tadpole.

A Novel Laser-Based Method for Studying Optic Nerve Regeneration

Optic nerve regeneration is a major challenge in neurobiology due to the limited self-repair capacity of the mammalian central nervous system (CNS) and the inconsistency of traditional injury models.…

Fluorescence microscopy of sectioned tissue, showing the interface between the extensor digitorum longus muscle and the common peroneal nerve in the adult rat. Regenerative peripheral nerve interface (RPNI) at 2 weeks. Image acquired using Mica. Stained for nuclei (blue), neurofilaments (green) and S100B (red). Image courtesy of Dr. Aaron Lee, Department of Bioengineering (Lab of Dr. Rylie Green), Imperial College London.

How to Image Axon Regeneration in Deep Muscle Tissue

This study highlights Dr. Aaron Lee’s research on mapping nerve regeneration in muscle grafts post-amputation. Limb loss often leads to reduced quality of life, not only from tissue loss but also due…

5 hour time-lapse maximum intensity projection of a zebrafish embryo along the z-axis at 3 days post fertilization. Left: microglia cells. Right: bright field channel. Courtesy of Prof. Francesca Peri, University of Zurich, Switzerland.

Capturing Developmental Dynamics in 3D

This application note showcases how the Viventis Deep dual-view light sheet microscope was successfully used by researchers for exploring high-resolution, long-term imaging of 3D multicellular models…

Some 2D measurements, e.g., lengths and areas, made on a PCB sample with a Leica measurement microscope using the Enersight software.

How to Select the Right Measurement Microscope

With a measurement microscope, users can measure the size and dimensions of sample features in both 2D and 3D, something crucial for inspection, QC, failure analysis, and R&D. However, choosing the…

A fruit fly (Drosophila melanogaster) observed with an Ivesta 3 stereo microscope during fly pushing (sorting of the flies). The scale bar length is 1 mm. Image courtesy of M. Benton, EMBL, Heidelberg, Germany.

A Guide to Using Microscopy for Drosophila (Fruit Fly) Research

The fruit fly, typically Drosophila melanogaster, has been used as a model organism for over a century. One reason is that many disease-related genes are shared between Drosophila and humans. It is…

这些图像说明，要捕捉特定细胞中的所有 gH2Ax 病灶并进行精确计数，用多个三维光切片方法实现。

罕见疾病 CRISPR 疗法的开发与风险解除

Fyodor Urnov博士和Sadik Kassim博士最初是在ASGCT 2025会议上作这一按需演讲的，演讲的重点是遗传医学中的一个关键挑战：如何将CRISPR疗法从单一疾病解决方案扩展到平台方法，特别是针对罕见的儿科遗传疾病。Urnov 博士展示了由 Matthew Kan 博士领导的创新基因组研究所的工作，这是 IGI-Danaher Beacon for CRISPR Cures…

Example of calibrating a microscope at a higher magnification value using a stage micrometer.

显微镜测量校准：为什么要校准以及如何校准

显微镜校准可确保用于检测、质量控制 (QC)、故障分析和研发 (R&D) 的测量结果准确一致。本文介绍了校准步骤。使用参照物进行校准可获得可重复的结果，并有助于确保与准则和标准一致。为获得准确一致的结果，建议校准显微镜并定期检查。如有需要，可向校准专家寻求支持。

Trichomonas parasite in mouse gut, rOTO Embedding and serial sections for array tomography imaging. Sample courtesy Isabelle Guerin-Bonne, Low Kay En, Electron Microscopy Unit, Yong loo Lin School of Medicine, National University of Singapore

用于三维生物成像的集成连续切片与冷冻电镜工作流程

本场网络研讨会探讨了集成化工具如何支持从样品制备到图像分析的电子显微镜全流程。专家Andreia Pinto博士、Adrian Boey博士与Hoyin Lai博士将介绍UC Enuity超薄切片机和Aivia图像分析平台，并演示这些工具如何同时适用于常温与低温实验环境。会议内容包含阵列断层成像、基于深度学习的图像分割、以及生物成像中cryo-lift-out工作流程的实际案例解析。

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