显微镜知识库

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

Single cells collected via laser microdissection as part of the Deep Visual Proteomics workflow.

AI meets Deep Visual Proteomics (DVP) to Advance Disease Research

In this webinar, Dr. Andreas Mund introduces Deep Visual Proteomics (DVP) – a cutting-edge platform that integrates AI-powered tissue modeling with spatially resolved, untargeted proteomics. He…

U2OS cells transfected with an Mx1-GFP plasmid (signal enhanced using Alexa Fluor 488-conjugared anti-GFP antibody) and co-stained for nuclear DNA (Hoechst 33342), microtubules (Alexa 555) and F-actin (ATTO 643). Image was captured on Mateo FL.

Microscopy and AI Solutions for 2D Cell Culture

This eBook explores the integration of microscopy and AI technologies in 2D cell culture workflows. It highlights how traditional imaging methods—such as brightfield, phase contrast, and…

Cell DIVE multiplexed image of FFPE tissue section from human invasive ductal carcinoma (IDC)

人工智能驱动的乳腺癌研究多重染色成像空间分析工具

乳腺癌（BC）是女性因癌症死亡的主要原因，研究查肿瘤微环境（TME）对于阐明肿瘤进展机制至关重要。利用超多标染色空间蛋白质组学技术系统地绘制肿瘤微环境图谱可以提高精准免疫肿瘤学的能力。在这里，我们将基于人工智能的高倍空间分析应用于BC组织，研究免疫细胞类型和生物标记物，从而深入了解受免疫疗法反应的TME分子机制。

偏振光显微镜影像图集

偏振光显微镜（又称为偏光显微镜）是一种应用于不同领域的重要方法，包括研究和质量保证。它不仅仅是在高倍率和高分辨率下产生图像，这通常是用普通光学显微镜完成的。通过检查样本的形状、结构、颜色、双折射和进一步的光学性质，可以获得有关样本结构、光学性质和成分的附加信息。

Artificial Intelligence (AI) segmentation used in conjunction with LMD to increase discovery throughput.

Biomarker Discovery with Laser Microdissection

Explore the potential of spatial proteomics workflows, such as Deep Visual Proteomics (DVP), to decipher pathology mechanisms and uncover druggable targets. Altered protein expression, abundance, or…

Image of roundworm C. elegans acquired with a M205 FA fluorescence automated stereo microscope in combination with Rottermann contrast. Areas labelled with mCherry are seen as reddish purple.

A Guide to C. elegans Research – Working with Nematodes

Efficient microscopy techniques for C. elegans research are outlined in this guide. As a widely used model organism with about 70% gene homology to humans, the nematode Caenorhabditis elegans (also…

Optic nerve regeneration in the Xenopus laevis tadpole.

A Novel Laser-Based Method for Studying Optic Nerve Regeneration

Optic nerve regeneration is a major challenge in neurobiology due to the limited self-repair capacity of the mammalian central nervous system (CNS) and the inconsistency of traditional injury models.…

Some 2D measurements, e.g., lengths and areas, made on a PCB sample with a Leica measurement microscope using the Enersight software.

How to Select the Right Measurement Microscope

With a measurement microscope, users can measure the size and dimensions of sample features in both 2D and 3D, something crucial for inspection, QC, failure analysis, and R&D. However, choosing the…

这些图像说明，要捕捉特定细胞中的所有 gH2Ax 病灶并进行精确计数，用多个三维光切片方法实现。

罕见疾病 CRISPR 疗法的开发与风险解除

Fyodor Urnov博士和Sadik Kassim博士最初是在ASGCT 2025会议上作这一按需演讲的，演讲的重点是遗传医学中的一个关键挑战：如何将CRISPR疗法从单一疾病解决方案扩展到平台方法，特别是针对罕见的儿科遗传疾病。Urnov 博士展示了由 Matthew Kan 博士领导的创新基因组研究所的工作，这是 IGI-Danaher Beacon for CRISPR Cures…

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