医疗

医疗

医疗

探索专为神经外科、眼科、整形外科、耳鼻喉科和牙科 HCP 量身定制的科学和临床综合资源,包括行业洞见、临床案例研究和专题研讨会。重点突出手术显微镜的最新进展,让您了解AR荧光、三维可视化和术中OCT成像等尖端技术如何赋能复杂手术决策的信心和精准操作。
C. elegans embedded in Lowicryl® HM20; pharynx showing red fluorescence (mCherry). The overview shows a front view onto the resin capsule formed by the bottom of a flow-through chamber of the EM AFS2. The capsule was pretrimmed manually. The blockface was trimmed automatically using the AutoTrim function of UC Enuity guided by fluorescence of the worm. Edge length of both squares in relation to the images is 250 µm.

How Fluorescence Guides Sectioning of Resin-embedded EM Samples

Electron microscopes, including transmission electron microscopes (TEM) and scanning electron microscopes (SEM), are widely utilized to gain detailed structural information about biological samples or…
嵌入在Epon环氧树脂中的秀丽隐杆线虫,与四氧化锇对比。树脂块经手工预修整。

如何通过自动化超薄切片技术节省时间与样本

本文阐述了如何利用树脂包埋电镜样本的 3D micro-CT 数据,在切片前将样本修整至预设目标平面。采用Leica UC Enuity 系统的交互式自动化方案,可显著节省时间、减少样本损耗及缩短新手用户的培训周期。
Complete camera overview of EM grid recorded with 3 channels. Inserts displaying the positions, where superresolved 3D confocal images were recorded. 3D renderings of these positions are shown in the zoomed inserts. Fluorescence channels (nuclei by Hoechst, blue; mitochondria by MitoTracker Green, green; lipid Droplets by Bodipy and Crimson Beads, red). Width of a grid square is 90 ?m, width of a grid bar is 35 ?m. Samples kindly provided by Ievgeniia Zagoriy, Mahamid-Group, EMBL Heidelberg, Germany.

从显微镜到电镜:完整的冷冻光电联用工作流程

在题为“多模态玻璃化征程,从实验台到电子显微镜的冷冻关联工作流程”的网络研讨会上,专家团队(Edoardo D'Imprima、Zhengyi Yang、Andreia Pinto 和 Martin…
Block-face created by automatic trimming under fluorescence. Mammalian cells of interest, stained with CellTrackerTM Green are visualized within the block-face using the UC Enuity equipped with the stereo microscope M205 FA. In the background a carbon finder grid in black is visible. All samples in the article are created by Felix Gaedke, PhD, CECAD, Cologne, Germany.

如何在块面中自动获取感兴趣的荧光细胞

本文介绍了使用超薄切片超薄切片机自动修整修块功能,获取树脂块面中带有荧光信号的细胞结构。我们展示了如何使用配置有体视显微镜 M205 FA 的超薄切片超薄切片机 UC Enuity ,来识别感兴趣的荧光细胞,如何自动修整包含细胞的块面,以及如何在切片中观察细胞而无需转移到外部显微镜。
UC Enuity

通过自动切片改善您的超薄切片工作流程

在不断发展的电镜样品制备领域,保持领先地位至关重要。这个网络研讨会提供了关于超薄切片最新进展的重要见解,这些进展可以显著增强您实验室的能力。

材料科学样本制备方法的工作流程解决方案

本手册介绍并解释了材料科学样本制备最常用的样本制备方法的工作流程解决方案:
Camera image during auto alignment. The feedback lines indicate if the correct edges in the image are detected. Green: Vertical center line; Magenta: Upper edge of the light gap; White: Lower edge of the light gap (not visible here, falling together with red line); Red: Knife edge; Blue: Left and right edge of the block face being automatically detected.

高质量超薄切片:样品与切片刀自动对齐

超薄切片技术是获取样品切片的最常用方法。在室温条件制备时,将样品小块嵌入环氧树脂中,然后通过修剪去除多余的树脂,并使用玻璃刀或金刚石刀将样品切成厚度为50-100纳米之间的薄片。
Section ribbons with increasing section thickness - silver to purple ending in blue sections.

超薄获得高质量的超薄切片

UC Enuity能够应对这一挑战,提供厚度一致且高质量的切片。其用户友好的设计不仅简化了研究过程,还提高了可重复性,使研究者对所得到的结论更有信心。
Material sample with a large height, size, and weight being observed with an inverted microscope.

工业应用中倒置显微镜相较于正置显微镜的五大优势

使用倒置显微镜时,您需要从下方观察样本,因为倒置显微镜的光学元件位于样本下方,而使用正置显微镜时,您需要从上方观察样本。一直以来,倒置显微镜主要用于生命科学研究,因为重力将样本沉入含有水性溶液的托座底部,从上方则无法观察到太多内容。但近段时间以来,倒置显微镜在工业应用中也变得越来越流行。我们现在一起来了解倒置显微镜在工业应用中的优势。
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