![[Translate to chinese:] How is microscopy used in spatial biology - Teaserimage](/fileadmin/_processed_/0/f/csm_spatial-biology-ebook-tease_5ac6218fa3.jpg "[Translate to chinese:] How is microscopy used in spatial biology? A microscopy guide.")
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Complex Made Simple: Antibodies in Multiplexed Imaging
Build panels, plan studies, and get the most from precious reagents using this antibody multiplexing guide from Leica Microsystems
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什么是膜片钳技术？
离子通道的生理学一直是神经科学家感兴趣的一个重要话题。诞生于1970年代的膜片钳技术开启了电生理学家的新时代。它不仅可以对整个细胞进行高分辨率电流记录，还可以对切下的细胞膜片进行高分辨率电流记录。甚至可以研究单通道事件。然而，由于需要复杂且高灵敏的设备，广泛的生物学背景和高水平的实验技能，电生理学仍然是最具挑战性的实验室方法之一。
![[Translate to chinese:] Neurons imaged with DIC contrast.](/fileadmin/_processed_/3/1/csm_Neurons_imaged_in_DIC_abb6a3742b.jpg "[Translate to chinese:] Neurons imaged with DIC contrast.")
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![[Translate to chinese:] Use of GLOW800 Augmented Reality during AVM surgery. Image courtesy of Prof. Philippe Bijlenga.](/fileadmin/_processed_/f/9/csm_Use_of_GLOW800_Augmented_Reality_during_AVM_surgery_29e3a7ab40.jpg "[Translate to chinese:] Use of GLOW800 Augmented Reality during AVM surgery. Image courtesy of Prof. Philippe Bijlenga.")
动脉瘤夹闭和AVM手术中的现实荧光
多数神经血管手术是脑外科手术。保护大脑非常重要。脑血管也至关重要，并且闭塞时间有限。在徕卡神经可视化峰会上，Philippe Bijlenga教授分享了他使用GLOW800增强现实荧光技术执行动脉瘤夹闭术和AVM手术的经验以及此方法的优势。
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![[Translate to chinese:] Masson-Goldner staining of a hedgehog brain slice.](/fileadmin/_processed_/c/0/csm_Masson-Goldner_staining_of_hedgehog_brain_slice_dd9e8977dd.jpg "[Translate to chinese:] Masson-Goldner staining of a hedgehog brain slice.")
如何利用单个系统对组织学荧光样品进行成像
在本集MicaCame中，主持人Lynne Turnbull和Patric Pelzer将带您探寻生物样本染色的历史之旅。他们将解释为什么您通常必须选择为组织学样品或荧光样品选择特定的系统，以及如何利用新的成像技术克服这一点。
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![[Translate to chinese:] The Emspira 3 digital microscope offers what users need for comprehensive visual inspection, including comparison, measurement, and documentation sharing.](/fileadmin/_processed_/6/d/csm_Emspira_3_digital_microscope_b95e1027fe.jpg "[Translate to chinese:] The Emspira 3 digital microscope offers what users need for comprehensive visual inspection, including comparison, measurement, and documentation sharing.")
数码检测显微镜的工业应用
本文讨论了在选择用于显微分析和质量控制（QC）以及故障分析（FA）和研发（R&D）的数码显微镜之前，用户应当考虑的因素。关键在于需要事先充分了解汽车、电子、机械工程和医疗设备等行业的应用要求和用户需求。显微镜解决方案不仅应当帮助用户实现高效、可靠的显微分析、QC、FA以及研发工作，还应当易于操作、满足用户需求，同时方便报告并分享结果。
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Going Beyond Deconvolution
Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
![[Translate to chinese:] Inspection microscope image of a printed circuit board (PCB) taken with a ring light (RL) and near vertical illumination (NVI).](/fileadmin/_processed_/2/7/csm_PCB_taken_with_a_ring_light_and_near_vertical_illumination_744697ca43.jpg "[Translate to chinese:] Inspection microscope image of a printed circuit board (PCB) taken with a ring light (RL) and near vertical illumination (NVI).")