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显微镜科学与教学知识中心

显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记,你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区,分享您的专业知识!
Masson-Goldner staining of a hedgehog brain slice.

How to Image Histological and Fluorescent Samples with One System

VIDEO ON DEMAND - How to image histological and fluorescent samples with one system. FluoSync, the new technology embedded into Mica enables the imaging of both histological staining and fluorescence…
[Translate to chinese:] The Emspira 3 digital microscope offers what users need for comprehensive visual inspection, including comparison, measurement, and documentation sharing.

数码检测显微镜的工业应用

本文讨论了在选择用于显微分析和质量控制(QC)以及故障分析(FA)和研发(R&D)的数码显微镜之前,用户应当考虑的因素。关键在于需要事先充分了解汽车、电子、机械工程和医疗设备等行业的应用要求和用户需求。显微镜解决方案不仅应当帮助用户实现高效、可靠的显微分析、QC、FA以及研发工作,还应当易于操作、满足用户需求,同时方便报告并分享结果。

Going Beyond Deconvolution

Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
[Translate to chinese:] Inspection microscope image of a printed circuit board (PCB) taken with a ring light (RL) and near vertical illumination (NVI).

工业应用的显微镜照明

本文旨在为使用显微镜检测的用户提供实用的建议,帮助他们为零件或组件观察选择最佳照明或照明系统
[Translate to chinese:] Image of MDCK (Madin-Darby canine kidney) cells taken with phase contrast.

相差和显微镜

相差是一种光学显微镜技术,用于增加未染色样本的对比度。未染色样本的结构,例如活细胞或其细胞器,在明场照明下观察时可能显得模糊,甚至变得透明。
[Translate to chinese:] Tumor resection leveraging 5-ALA fluorescence. Image courtesy of Dr. Carla Reizinho.

高级别胶质瘤的手术治疗

胶质瘤是中枢神经系统最常见的一种原发性肿瘤,其中50%为恶性胶质母细胞瘤。胶质瘤治疗难度高,同时因为容易扩散和浸润,预后不佳,导致确诊后的中位生存期仅为12到16个月。2021年举行的神经可视化峰会汇集了全球各地的神经外科医生,医生在这场独特活动上,分享了她在高级别神经胶质瘤手术治疗方面的经验,尤其是最大范围安全切除的术中策略。

How to Radically Simplify Workflows in Your Imaging Facility

VIDEO ON DEMAND - How to radically simplify imaging workflows and generate meaningful results with less time and effort using a highly automated microscope that unites widefield and confocal imaging.

浸没式物镜

要用显微镜在高倍镜下检查试样标本,有许多因素需要考虑。其中包括分辨率、数字光圈数值孔径(NA)、物镜的工作距离以及物镜前透镜采集图像所用介质的折射率。在这篇文章中,我们将简要地看一下如何使用浸泡介质在盖玻片和物镜前透镜之间利用浸泡介质帮助提高NA和分辨率。此外,我们还将考虑空气和构成载玻片和、盖玻片的空气和玻璃的折射率,以及当光从一种介质传播到另一种介质时,如何使用浸没介质部分地减少失错配。此外,…
Raw widefield and THUNDER image of Drosophila follicles. Image courtesy of M. Khoury and D. Bilder, University of California, Berkeley, USA.

Investigating Epithelial Morphogenesis and the Growth of Epithelia

This article shows how rapidly acquired THUNDER images of Drosophila follicles or egg chambers help distinguish apicobasal proteins when studying epithelial morphogenesis for cancer research.
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