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Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches
The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.
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A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
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How to Quantify Changes in the Metabolic Status of Single Cells
Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed.
Now,…
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Adding Dimensions to Multiplex Molecular Imaging
Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of complex…
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How FLIM Microscopy Helps to Detect Microplastic Pollution
The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…
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How to Uncover Hidden Dimensions in Research with Lifetime Imaging
Learn how fluorescence lifetime imaging adds information depth to your confocal experiments and reveals novel insights that are difficult or impossible to discover using conventional intensity-based…
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Development of Fluorescence Lifetime Imaging Microscopy (FLIM) and its Relevance for Functional Imaging
Prof. Ammasi Periasamy, Director, Keck Center for Cellular Imaging, University of Virginia, was interviewed by Dr. Giulia Ossato, Product Manager functional imaging, during Leica Microsystems Meets…
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DIVE Multiphoton Microscope Image Gallery
Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…
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Widefield Calcium Imaging with Calcium Indicator Fura2
In eukaryotic cells Ca2+ is one of the most widespread second messengers used in signal transduction pathways. Intracellular levels of Ca2+ are usually kept low, as Ca2+ often forms insoluble…