显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

Image of a Siemens star, where the diameter of the 1st black line circle is 10 mm and the 2nd is 20 mm, taken via an eyepiece of a M205 A stereo microscope. The rectangles represent the field of view (FOV) of a Leica digital camera when installed with various C-mounts (red 0.32x, blue 0.5x, green 0.63x).

30000:1放大率到底意味着什么？

关于光学显微镜性能的一个重要标准是放大率。本报告将为数字显微镜用户提供有用的指南，以确定放大率值的有用范围。

Image of an onion flake taken with a basic Leica compound microscope after it was tested for resistance to fungus and mold growth following part 11 of the ISO 9022 standard.

ISO 9022 Standard Part 11 - Testing Microscopes with Severe Conditions

This article describes a test to determine the robustness of Leica microscopes to mold and fungus growth. The test follows the specifications of the ISO 9022 part 11 standard for optical instruments.

Stereo microscopes are often considered the workhorses of laboratories and production sites.

考虑采购体视显微镜时的关键因素

体视显微镜通常是实验室或生产现场“主力”。用户需要花费数小时通过目镜来检查、观察、记录或解剖样本。仔细评估哪些相关应用需要用到体视显微镜，是确保长期满意使用的关键所在。决策者们需要确保自己能够完全依照自己的需求来定制仪器。为帮助用户能更好的选择适合自己的体视镜，本文介绍了几个主要考虑的因素。

[Translate to chinese:] The principle of the FusionOptics technology: Of the two separate beam paths (1), one provides depth of field (2) and the other high resolution (3). In the brain, the two images of the sample are merged into a single, optimal 3D image (4).

FusionOptics融合光学（徕卡专利技术）高分辨率和大景深结合

人类对视觉环境的感受，80%通过视觉感知获得。如果没有空间视觉，我们几乎无法确定方向。我们的视觉皮质和大脑皮层通过复杂的过程，巧妙地处理着从眼睛看到的图像信号。最近几十年，神经科学已经大量了解该过程。故由徕卡显微系统、苏黎世大学神经信息学研究所和瑞士联邦理工学院共同开展的一项研究，显示了我们的大脑如何灵活有效地结合视觉信号创造出最佳空间图像。研究结果为立体显微技术创新奠定了基础，该创新从分辨率和聚…

[Translate to chinese:] Strain from using a microscope can lead to musculoskeletal pain.

如何使显微镜工作场所符合人体工学

对于每天使用显微镜工具的人来说，显微镜具有重要影响。显微镜对人体有着很高的要求，需要我们集中注意力，运用肌肉从事大量稳定活动。在此次采访中，Leica Microsystems的高级产品经理Clinton Smith谈到了如何缓解可能出现的紧张和压力，如何创造符合人体工学的工作场所来帮助显微镜用户舒适地工作，以及如何提高生产率。

[Translate to chinese:] Inspection microscope image of a printed circuit board (PCB) taken with a ring light (RL) and near vertical illumination (NVI).

工业应用的显微镜照明

本文旨在为使用显微镜检测的用户提供实用的建议，帮助他们为零件或组件观察选择最佳照明或照明系统

Elevate your Inspection Workflow for Medical Devices

This webinar will demonstrate a smart device for traceable microscopy that helps you achieve more consistent visual inspection of medical devices across multiple users. Additionally, this device…

Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture. Image courtesy of Dr. Selina Keppler, Munich, Germany.

Image Gallery: THUNDER Imager

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…

Mouse retina was fixed and stained by following reagents: anti-CD31 antibody (green): Endothelia cells, IsoB4 (red): Blood vessels, and microglia anti-GFAP antibody (blue): Astrocytes Sample courtesy by Jeremy Burton, PhD and Jiyeon Lee, PhD, Genentech Inc., South San Francisco, USA. Imaged by Olga Davydenko, PhD (Leica). Imaged with a THUNDER Imager 3D Cell Culture.

An Introduction to Computational Clearing

Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…