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![[Translate to chinese:] Preparation of an IC-chip cross section: grinding and polishing of the chip cross section.](/fileadmin/_processed_/3/b/csm_Grinding_polishing_EM_TXP_91d5c1584d.jpg "[Translate to chinese:] Preparation of an IC-chip cross section: grinding and polishing of the chip cross section.")
电子产品制造截面分析
本文将讨论印刷电路板 (PCB) 和总成 (PCBA)、集成电路 (IC) 和电池组件的横截面为什么对质量控制 (QC)、故障分析 (FA) 和研发 (R&D) 有效，以及如何制备这些横截面。
![[Translate to chinese:] Water Flea Daphnia imaged by Electron Microscopy. Courtesy of Mag. Dr. Gruber, University of Vienna, Austria](/fileadmin/_processed_/e/d/csm_EM_Application_Water_Flea_Daphnia_7abc89f4fb.jpg "[Translate to chinese:] Water Flea Daphnia imaged by Electron Microscopy. Courtesy of Mag. Dr. Gruber, University of Vienna, Austria.")
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![[Translate to chinese:] Material sample with a large height, size, and weight being observed with an inverted microscope.](/fileadmin/_processed_/f/4/csm_Inverted_microscope_with_large_sample_crop_04c3257c81.jpg "[Translate to chinese:] Material sample with a large height, size, and weight being observed with an inverted microscope.")
工业应用中倒置显微镜相较于正置显微镜的五大优势
使用倒置显微镜时，您需要从下方观察样本，因为倒置显微镜的光学元件位于样本下方，而使用正置显微镜时，您需要从上方观察样本。一直以来，倒置显微镜主要用于生命科学研究，因为重力将样本沉入含有水性溶液的托座底部，从上方则无法观察到太多内容。但近段时间以来，倒置显微镜在工业应用中也变得越来越流行。我们现在一起来了解倒置显微镜在工业应用中的优势。
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![[Translate to chinese:] EBSD grain size distribution of the cross section of a gold wire within a silicon matrix from inside a CPU (central processing unit of a computer). The grains are highlighted with arbitrary colors.](/fileadmin/_processed_/d/0/csm_EBSD_grain_size_distribution_of_the_cross_section_of_a_gold_wire_58f1fb038c.jpg "[Translate to chinese:] EBSD grain size distribution of the cross section of a gold wire within a silicon matrix from inside a CPU (central processing unit of a computer). The grains are highlighted with arbitrary colors.")
非聚焦离子束研磨制备高质量EBSD样品
背散射电子衍射技术（EBSD）能在样本表面上方数十纳米处生成电子衍射，因而它又称为一种“表面”技术。采用这种方法生成EBSD图案时，样本表面不会遭受任何损坏。本文中，我们介绍了使用EBSD高效抛光两个通过非聚焦离子束研磨制备的高难度样本的案例。
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![[Translate to chinese:] Image of murine-brain tissue showing a region removed with UV laser microdissection.](/fileadmin/_processed_/a/4/csm_Murine-brain_tissue_showing_a_region_removed_with_UV_LMD_e02d1597b3.jpg "[Translate to chinese:] Image of murine-brain tissue showing a region removed with UV laser microdissection.")
不同组织样品制备方法的RNA质量
本文介绍了样品制备过程和紫外激光显微切片（UV LMD）对小鼠脑组织冷冻切片RNA质量的影响。为在提取RNA时获取良好的结果，从高品质组织开始并在处理前后检查RNA质量至关重要。RNA完整性指数（RIN）以1到10的等级标准来显示样品质量。简言之，RIN数值越高，RNA质量越高。这项研究结果表明，可以利用紫外激光显微切片技术，可以从单个组织细胞中获取品质稳定的RNA。
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How Industrial Applications Benefit from Fluorescence Microscopy
Watch this free webinar to know more about what you can do with fluorescence microscopy for industrial applications. We will cover a wide range of investigations where fluorescence contrast offers new…
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冷冻光电联用（Cryo-CLEM）之旅
本文主要介绍Cryo-CLEM技术及其为科学家带来的便益。此外，还特别说明了一些相关文献。
近期在冷冻电子显微镜工作流程领域取得的技术进步，让我们能够获取到细胞蛋白质社会学的3D数据，其分辨率更是达到前所未有的1纳米以下。工作流程中有一个步骤，需要从样品获取目标位置纳米级分辨率的图像，而要得到这样的结果，就需要用到冷冻光学显微镜。这种显微镜如果用于低温电子显微镜工作流程，通常就称为Cryo…
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Fast, High-quality Vitrification with the EM ICE High Pressure Freezer
The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…
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Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…