Leica Microsystems: 显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

How To Create EDOF (Extended Depth of Focus) Images

Watch this video to see how you can rapidly record sharp optical microscope images of samples with a large height variation. This is done with the optional Extended Depth of Focus (EDOF) function of…

Schematic graph of the light path in a Spalt-Ultramikroskop.

Confocal and Light Sheet Imaging with STELLARIS DLS

Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the…

FusionOptics in Neurosurgery and Ophthalmology – for a Larger 3D Area in Focus

Neurosurgeons and ophthalmologists deal with delicate structures, deep or narow cavities and tiny structures with vitally important functions. A clear, three-dimensional view on the surgical field is…

Object planes of the Greenough stereomicroscope with depth of field range.

如何形成清晰的图像

在显微镜检查中，景深常被看做经验参数。在实际操作中，会根据数值孔径、分辨率和放大率之间的相关性确定该参数。为了获得最佳视觉效果，现代显微镜的调节设备在景深和分辨率之间实现了最佳平衡，这两个参数在理论上呈负相关。