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![[Translate to chinese:] Five-color FLIM-STED](/fileadmin/_processed_/a/3/csm_5-color_FLIM-STED_9d88340e60.jpg "[Translate to chinese:] Five-color FLIM-STED")
采用单损耗激光的五色FLIM-STED显微镜
网络研讨会，内容涉及使用单一损耗激光和荧光寿命phasor分离技术的五色STED技术。
A Versatile Palette of Fluorescent Probes
Researchers at the Max Planck Institute for Medical Research in Heidelberg have developed a general strategy to synthesize live-cell compatible fluorogenic probes, and the result are the new MaP (Max…
Benefits of Combining STED and Lifetime
In this interview, Professor Alberto Diaspro talks about the advantages of the White Light Laser and the TauSTED capabilities of STELLARIS 8 STED. He speaks about his experience with the confocal…
Effects of Clearing Media on Tissue Transparency and Shrinkage
This study comprehensively evaluates the effects of different clearing media on tissue transparency and shrinkage by comparing freshly dissected dipteran fly brains with their cleared equivalents.…
Super-Resolution Microscopy Image Gallery
Due to the diffraction limit of light, traditional confocal microscopy cannot resolve structures below ~240 nm. Super-resolution microscopy techniques, such as STED, PALM or STORM or some…
Regulators of Actin Cytoskeletal Regulation and Cell Migration in Human NK Cells
Dr. Mace will describe new advances in our understanding of the regulation of human NK cell actin cytoskeletal remodeling in cell migration and immune synapse formation derived from confocal and…
Nanoscopy meets Lifetime: Introducing TauSTED
TauSTED combines the optical signals from STED and the photophysical information from the fluorescence lifetime at unprecedented speeds. This approach to STED uses phasor analysis in a novel way.…
The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS SP8 STED 3X nanoscope from Leica Microsystems. It…
Extending Nanoscopy Possibilities with STED and exchangeable fluorophores
When it comes to STED Nanoscopy, keeping high signal-to-noise is key to achieve the best possible resolution in fixed and living cells. This can be challenging in the case of experiments in 3D and/or…
See More Than Just Your Image
Despite the emergence of new imaging methods in recent years, true 3D resolution is still achieved by Confocal Laser Scanning Microscopy (CLSM). Through a combination of novel, extremely fast scanning…
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