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What are the Challenges in Neuroscience Microscopy?

eBook outlining the visualization of the nervous system using different types of microscopy techniques and methods to address questions in neuroscience.

[Translate to chinese:] Raw widefield and THUNDER image of calcium transients in Drosophila embryos. Courtesy A. Carreira-Rosario, Clandinin laboratory, California, USA.

Central Nervous System (CNS) Development and Activity in Organisms

This article shows how studying central nervous system (CNS) development in Drosophila-melanogaster embryos expressing a GCaMP calcium indicator in the neurons can be improved with a THUNDER Imager.

[Translate to chinese:] Raw widefield (left) and Computationally Cleared image (right) of mouse neuromuscular junctions acquired with a THUNDER Imager. Courtesy of A. Yung and M. Krasnow in California, USA.

呼吸、发声以及喉部疾病的神经基础

这项研究显示了使用THUNDER Imager对于小鼠完整喉肌进行成像的优势，这对于研究癌症和喉部疾病具有重要作用。喉部对呼吸和发声至关重要。相关癌症和疾病的研究通常以小鼠作为研究模型。相比传统的宽场显微镜成像，THUNDER Imager通过计算光学去除非焦面信号后，可以更好地区分小鼠喉肌运动神经元中经免疫荧光染色的神经肌肉接头和神经纤维细丝。

Going Beyond Deconvolution

Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…

Raw widefield and THUNDER image of Drosophila follicles. Image courtesy of M. Khoury and D. Bilder, University of California, Berkeley, USA.

Investigating Epithelial Morphogenesis and the Growth of Epithelia

This article shows how rapidly acquired THUNDER images of Drosophila follicles or egg chambers help distinguish apicobasal proteins when studying epithelial morphogenesis for cancer research.

Raw widefield and THUNDER image of GFP-tagged zebrafish fin. Courtesy of Jason Ear lab at Cal Poly Pomona, California, USA.

Diseases Linked to Scaffold Proteins and Signaling

This article shows how diseases related to scaffold proteins and protein signaling can be studied in zebrafish models efficiently with a THUNDER Imager.

Extended depth of field reconstruction image of a whole human pancreas islet showing fluorescence signals from insulin (green), glucagon (red), an IL17 cytokine (magenta), and nuclei (blue).

Understanding Better the Onset of Diabetes

This article shows how Interleukin-17 (IL-17) proinflammatory cytokine proteins in human pancreatic islets can be studied efficiently with a THUNDER Imager.

Maximum intensity projection of undecalcified mouse bone tissue expressing GFP (green) and tdTomato (red) and stained with Hoechst 33342 (blue). Imaged using a THUNDER Imager Tissue: A) raw data and B) with ICC.

Localizing Bone Stem Cells In Vivo

This article demonstrates how undecalcified mouse bone samples can be investigated in detail more easily and rapidly with a cryohistological method used in combination with a THUNDER Imager Tissue and…

Fluorescently stained mouse tibia, imaged with a THUNDER Imager 3D Tissue. Courtesy of Dr. Anjali Kusumbe, University of Oxford, UK.

High-resolution 3D Imaging to Investigate Tissue Ageing

Award-winning researcher Dr. Anjali Kusumbe demonstrates age-related changes in vascular microenvironments through single-cell resolution 3D imaging of young and aged organs.