Leica Microsystems: 显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

宽场显微镜简介

‘宽场显微镜’是最基本的显微镜技术之一。其根本上是将整个感兴趣的样本暴露于光源下，由观察者或摄像头（也可连接到计算机显示器）获得图像的技术。

无像差显微镜光学组件的互动优化

光学显微镜旨在放大肉眼不可见的物体。为此需要采用高品质光学器件来获得优秀的分辨率。但是，所有光学组件都会对光路中的光线带来负面的影响，最终导致像差。本文将重点介绍此过程中涉及的光学元件及其物理参数。在此基础上，本文对减少像差的方法原理进行了一次历史概述。结果表明，将显微镜看作一个整体系统有助于协调其各个组件并获得最佳微观结果。

Multicolor image of a Confetti Mouse recorded with the new SP8 DIVE with the 4Tune spectral detector. The adaptation to the transgenic markers allows to achieve more contrast and depth for multi-color in vivo deep imaging. Sample courtesy of Jacco van Rheenen, University of Utrecht, NL.

About the Most Important Considerations When Imaging Deep Into Mouse Tissue

When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…

Primary Beam Splitting Devices for Confocal Microscopes

Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam…

[Translate to chinese:] Dronpa chromophore

荧光蛋白（FP）如GFP、YFP或DsRed都是可视化观察活细胞中细胞组分的强大工具。尽管如此，目前仍然会有传统FP达到极限的情形发生。普通FP无法观察特定兴趣蛋白中专有、空间有限的蛋白质群体，因为它们在整个细胞中都有表达。此时，光激活、光转化和光控开关荧光蛋白就登上了舞台。荧光套件中的成员可以从非荧光状态激活，可以改变发射光谱，甚至可以逆转式地“开启和关闭”。借助这些“光学荧光笔”，研究人员可以…

[Translate to chinese:] Pinhole diameter and diffraction pattern.

共聚焦显微镜针孔效应

在操作共聚焦显微镜，或在讨论这种装置的特性和参数时，我们不可避免地提到针孔及其直径。这篇简短的文章是针对那些没有足够时间钻研共聚焦显微镜的理论和细节但又想了解针孔效应的用户们来解释针孔的意义。

Comparison when observing a rodent model organism with a Greenough versus CMO (common main objective) stereo microscope for a task like surgery.

Rodent and Small-Animal Surgery

Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.

Navigating Through the Brain

One of the challenges of neurosurgery is orientation at the surgical site. When resecting tumors, removing arteriovenous malformations, or clipping aneurysms, surgeons often have to work near healthy…

[Translate to chinese:] Left: Tissue cells marked with an immunolabel (FITC) illuminated with wide-band UV excitation. Note the tissue structure with blue autofluorescence. Right: Same tissue and same immunostaining with FITC label illuminated with epi-illumination using narrow-band blue (490 nm) light. Note the increased image contrast (Ploem, 1967)

Milestones in Incident Light Fluorescence Microscopy

Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…