Leica Microsystems: 显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

STED imaging of mitochondria revealed cristae dynamics during fusion. A comparison of confocal (left) and STED (right) imaging.

用STED显纳镜定量显示线粒体的动态变化

线粒体是重要的细胞器，可产生支持生物细胞整个生命周期所需的能量。然而，传统的荧光显微镜分辨率不足以对线粒体嵴进行可视化。本研究证实了光学STED显纳镜能以纳米级分辨率长时间研究细胞内的生理过程。

Formulated Product Characterization with SRS Microscopy

Creams, pastes, gels, emulsions, and tablets are ubiquitous across a wide range of manufacturing sectors from pharmaceuticals and consumer health products to agrochemicals and paint. To improve…

[Translate to chinese:] SEM image of the full Li-NMC electrode sample, showing the two porous layers and the metal film at the center of the structure.

电池组件的横截面离子束研磨（锂电池与铅酸电池栅板）

深入了解锂电池系统需要高质量的表面处理，以评估其内部结构和形态。然而，快速简单地制备原始横截面可能由于所涉及材料的性质和电池结构而变得困难。多数材料系统通常使用切割、包埋、研磨、抛光等纯机械方法制备横截面。在这种情况下，单纯的机械制备不足以对电池进行高分辨率的SEM分析。

Fluorescently stained mouse tibia, imaged with a THUNDER Imager 3D Tissue. Courtesy of Dr. Anjali Kusumbe, University of Oxford, UK.

High-resolution 3D Imaging to Investigate Tissue Ageing

Award-winning researcher Dr. Anjali Kusumbe demonstrates age-related changes in vascular microenvironments through single-cell resolution 3D imaging of young and aged organs.

如何成功进行活细胞光电关联

Coral Life 提供了简化的活细胞 CLEM 解决方案，用于深入了解细胞成分随时间发生的结构变化。除了工作流程手册中描述的技术处理外，本文还提供了成功进行实验的其他知识。

How to Successfully Implement Coral Life

The live-cell  CLEM workflow allows you to capture dynamic information related to a relevant biological process as it happens and put these observations into their ultrastructural context. The Leica…

Advancing Cellular Ultrastructure Research

Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…

Proveo 8 with intraoperative OCT – a User Evaluation in an University Setting

Optical coherence tomography (OCT) makes structures in the eye visible that lie beneath the surface. When OCT is used intraoperatively, surgeons gain insight into possible pathological changes in the…

Cell DIVE已验证的抗体将使您对实验结果产生信心

Cell DIVE超多标组织成像分析整体解决方案包括经严格验证的350+抗体资源库，高灵敏度高特异性的应用于Cell DIVE循环染色成像中。抗体验证的方法可以帮助您找到合适的抗体以及最佳的实验条件，快速的让您开展超多标成像分析的实验。抗体库中的每种抗体都经过严格的三步验证过程，（a）评估在FFPE上的表现性能；（b）确定其最佳的染色条件以及是否可用作直标抗体；（c）探究由于Cell…