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THUNDER成像系统 Live Cell 和 3D Assay 活细胞培养显微成像系统

实时解构 3D 生物微观世界*

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THUNDER image of brain-capillary endothelial-like cells derived from human iPSCs (induced pluripotent stem cells) where cyan indicates nuclei and magenta tight junctions.

Rapid Check of Live Stem Cells in Cell-culture Inserts Set in Multi-well Plates

See how efficient imaging of live iPSC stem cells within cell-culture inserts set in a multi-well plate can be done to evaluate the cells using a THUNDER Imager. Just read this article.
[Translate to chinese:] Murine esophageal organoids (DAPI, Integrin26-AF 488, SOX2-AF568) imaged with the THUNDER Imager 3D Cell Culture. Courtesy of Dr. F.T. Arroso Martins, Tamere University, Finland.

如何深入了解类器官和细胞球模型

在本电子书中,您将了解3D细胞培养模型(如类器官和细胞球)成像的关键注意事项。探索创新型显微镜解决方案,来实时记录类器官和细胞球的动态成像过程。
[Translate to chinese:] Single cell datasets

利用 SPARCS 探索亚细胞空间表型

功能日益强大的显微镜可提供信息丰富的各种细胞表型数据。如果与深度学习的最新进展相结合,这将成为在基因筛选中读出感兴趣的生物表型的理想技术。在本网络讲座中,您将了解到空间分辨 CRISPR 筛选 (SPARCS),这是一种利用自动化高速激光显微切割技术在人类基因组尺度上揭示各种亚细胞空间表型的平台。
[Translate to chinese:] Brain organoid section (DAPI) acquired using THUNDER Imager Live Cell. Image courtesy of Janina Kaspar and Irene Santisteban, Schäfer Lab, TUM.

研究大脑健康的成像类器官模型

小胶质细胞是特化的脑驻留免疫细胞,在大脑发育、平衡和疾病中发挥着至关重要的作用。然而,到目前为止,模拟人脑环境与小胶质细胞之间相互作用的能力还非常有限。
[Translate to chinese:] Mouse cortical neurons. Transgenic GFP (green). Image courtesy of Prof. Hui Guo, School of Life Sciences, Central South University, China

显微镜如何帮助研究机械感受和突触通路

Tobi Langenhan教授使用显微镜研究突触蛋白质组合体,研究粘附性GPCR的机械感受特性,并了解蛋白质动力学及其空间相互作用。
Microscopy for neuroscience research

What are the Challenges in Neuroscience Microscopy?

eBook outlining the visualization of the nervous system using different types of microscopy techniques and methods to address questions in neuroscience.
[Translate to chinese:] Cancer cells

铁代谢在癌症进展中的作用

铁代谢在癌症发展和演进过程中发挥着重要作用,可以调节免疫反应了解铁离子如何影响癌症和免疫系统,有助于开发新的癌症治疗方法。
[Translate to chinese:] How is microscopy used in spatial biology - Teaserimage

显微镜如何应用在空间生物学中?一份显微镜指南

本电子书旨在探索显微镜中的关键空间生物学方法,例如多重成像技术,这个方法有助于将独立的细胞信息放入空间环境来分析。

Going Beyond Deconvolution

Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
Raw widefield and THUNDER image of Drosophila follicles. Image courtesy of M. Khoury and D. Bilder, University of California, Berkeley, USA.

Investigating Epithelial Morphogenesis and the Growth of Epithelia

This article shows how rapidly acquired THUNDER images of Drosophila follicles or egg chambers help distinguish apicobasal proteins when studying epithelial morphogenesis for cancer research.
Raw widefield and THUNDER image of GFP-tagged zebrafish fin. Courtesy of Jason Ear lab at Cal Poly Pomona, California, USA.

Diseases Linked to Scaffold Proteins and Signaling

This article shows how diseases related to scaffold proteins and protein signaling can be studied in zebrafish models efficiently with a THUNDER Imager.
[Translate to chinese:] Extended depth of field reconstruction image of a whole human pancreas islet showing fluorescence signals from insulin (green), glucagon (red), an IL17 cytokine (magenta), and nuclei (blue).

更好地了解糖尿病的发病过程

本文讨论了通过高对比度快速成像技术研究人胰岛标本中的白细胞介素-17(IL-17)促炎细胞因子蛋白。更好地了解糖尿病的发病机制,即糖尿病是如何开始的,是开发新疗法的关键。1型糖尿病(T1D)是一种自身免疫性疾病,患者体内产生胰岛素的胰腺β细胞会受到免疫系统的攻击。更多地了解 T1D 患者胰腺中的细胞因子有助于开发更好的疗法和治疗方法。
[Translate to chinese:] Chicken-embryo cross section at the level of the midbrain showing neural crest cells and cadherin-6B molecules. Raw widefield data (top) and THUNDER image (bottom).

神经嵴(NC)

本文主要讨论如何使用THUNDER Imager 3D Assay进行高清成像来助力鸡胚神经嵴(NC)发育的研究。在发育过程中,NC细胞的适当特化、迁移和分化对于防止颅面畸形和神经病变至关重要。使用具有computational clearing功能的THUNDER Imager对NC细胞上皮-间充质转化,以及钙粘蛋白-6B粘附分子的迁移过程进行清晰的成像,较之传统的宽场显微镜能够获得更多细节。
[Translate to chinese:] Raw widefield and THUNDER image of transversal mouse adult fiber lens section. Courtesy N. Houssin, Plagemen lab, Ohio State University, Columbus, USA.

眼部出生缺陷研究

本文讨论如何使用快速采集的宽场显微镜图像研究晶状体的形成和眼部出生缺陷。将小鼠晶状体用作模型,以研究眼睛细胞形态发生及细胞过程如何中断并导致发生缺陷。通过无模糊或离焦模糊的图像揭示了小鼠晶状体玻片内部深处的细节。这些图像是使用THUNDER Imager 3D Assay采集的。
THUNDER image of early prophase Drosophila germarium.

健康卵母细胞发育

本文讨论了如何通过THUNDER Imager活细胞成像系统,获取清晰、无模糊的图像来帮助研究果蝇健康卵母细胞生成的相关机制。一个鲜为人知的例子就是联会,它对染色体分离至关重要。THUNDER图像可以显示卵巢中卵母细胞的精细结构,这有助于更好地了解减数分裂和其他导致健康卵母细胞生成的过程。
Mouse whole-mount retina. Image courtesy of the Experimental Ophthalmology Group, University of Murcia, Spain.

快速、高灵敏度成像和人工智能辅助分析

The specificity of fluorescence microscopy allows researchers to accurately observe and analyze biological processes and structures quickly and easily, even when using thick or large samples. However,…
3D reconstruction of an isolated human islet

为活细胞成像创造新选择

对厚实的活体样本进行成像时,主要挑战之一是获得图像质量与组织完整性之间的平衡。长时间的图像采集期间,弱信号光会导致低信号水平,导致图像对比度低以及分割和分析困难。需要通过高剂量成像或高时间分辨率成像技术加强信号强度时,这一问题更加突出。一个常见问题是:我如果快速成像、一次完成,会不会造成样本过度漂白或者细胞死亡?
[Translate to chinese:] Raw widefield and THUNDER image of mouse brain showing D2-dopamine receptors.

D2多巴胺受体在神经元中的表达

本文讨论如何通过使用Instant Computational Clearing(ICC)的THUNDER Imager 3D Assay比传统宽场显微镜更加清晰地观察小鼠脑黑质中的D2多巴胺受体(D2R)。

视网膜相互作用的可视化助力眼部疾病的研究

本文描述通过使用THUNDER Imager 3D Cell Culture和Large Volume Computational Clearing(LVCC)技术高效地研究小鼠视网膜中内皮细胞、血管、小胶质细胞和星形胶质细胞之间的相互作用。
[Translate to chinese:] Whole mouse embryo widefield data and LVCC

脑神经发育

本文介绍了如何使用THUNDER Imager 3D Cell Culture和Large Volume Computational Clearing(LVCC)对小鼠胚胎快速、高对比度成像,实现了对轴突生长和脑神经发育的研究。许多在发育早期阶段损害神经回路发育的遗传性疾病被认为会对行为造成干扰。用小鼠模型研究早期神经发育的细胞变化、定义与人类疾病相似的行为及潜在发育机制,是非常困难的。
Left-hand image: The distribution of immune cells (white) and blood vessels (pink) in white adipose tissue (image captured using the THUNDER Imager 3D Cell Culture). Right-hand image: The same image after automated analysis using Aivia, with each immune cell color-coded based on its distance to the nearest blood vessel. Image courtesy of Dr. Selina Keppler, Munich, Germany.

精确分析宽视野荧光图像

利用荧光显微镜的特异性,即便是使用厚样品和大尺寸样品,研究人员也能够快速轻松地准确观察和分析生物学过程和结构。然而,离焦荧光会提高背景荧光,降低对比度,影响图像的精确分割。THUNDER 与Aivia 的组合可以有效解决这一问题。前者可以消除图像模糊,后者会使用人工智能技术自动分析宽视野图像,提高操作速度和精确性。下面,我们来详细了解下这一协作方法。

Save Time and Effort with AI-assisted Fluorescence Image Analysis

The powerful synergy of THUNDER and Aivia analyze fluorescence images with greater accuracy, even when using low light excitation.

Role of Mucins and Glycosylation in Dry Eye Disease

This article shows how fast, high-contrast, and sharp imaging of stratified human corneal epithelial cells with THUNDER imaging technology for dry eye disease (DED) research allows membrane ridges to…
Images of C2C12 cells which were stained with lamin B (magenta), Hoechst (blue), and yH2AX (yellow). A) raw widefield fluorescence image and B) THUNDER image after LVCC. The yellow foci indicate damaged DNA.

Skeletal Muscle Adaptation and Fibrotic Diseases

The mechanisms of how skeletal muscle adapts to fibrotic pathologies can be investigated more efficiently with fast, high-contrast imaging of C2C12 mouse myoblast cells which is described in this…
[Translate to chinese:] HeLa Kyoto cells (HKF1, H2B-mCherry, alpha Tubulin, mEGFP). Left image: Maximum projection of a z-stack prior to ICC and LVCC. Right image: Maximum projection of a mosaic z-stack after ICC and LVCC.

如何使用Coral Life(活细胞光电联用)改进活细胞成像

对于活细胞 CLEM 应用而言,光学显微镜成像是在正确的时间以正确的状态识别正确细胞的关键步骤。在本文中,徕卡专家就使用宽场系统的优势以及使用蓝宝石作为细胞培养基底时需要克服的障碍分享了他们的见解。

Optimizing THUNDER Platform for High-Content Slide Scanning

With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging technology are tested, while user trainability and…
C. elegans Gonades - THUNDER Imager  Adult hermaphrodit, Staining: blue - DAPI (Nucleus), green - SP56 (sperms), red - RME-2 (oocyte), mangenta - PGL-1 (RNA + protein granules) Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany

Physiology Image Gallery

Physiology is about the processes and functions within a living organism. Research in physiology focuses on the activities and functions of an organism’s organs, tissues, or cells, including the…
[Translate to chinese:] Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imagerwith a 2x 0.15 NA objective at 3.4x zoomwas used to produce this 425 μm Z-stack (26 positions), which is presented here as an Extended Depth of Field(EDoF)projection.

神经科学图像

神经科学通常使用显微镜来研究神经系统的功能和了解神经退行性疾病。
Pollen Flower - Taken with a 20x/0.8 objective, area of 6mm² with a depth of 100μm. 15 stitched tiles with 4 colors (DAPI/GFP/TRITC/Cy5) - a total of 13020 images. Video courtesy of James Marr, Leica Microsystems, USA

Developmental Biology Image Gallery

Developmental biology explores the development of complex organisms from the embryo to adulthood to understand in detail the origins of disease. This category of the gallery shows images about…
Images of a brain organoid derived from iPSCs acquired with a THUNDER Imager 3D Cell Culture. The cells were infected with the pAAV-hSyn-EGFP and pLX-hGFAP-mCherry virus. The image is the 36th plane cropped out of a 53 plane Z-stack volume. Shown are both the A) raw widefield image and B) the same image after Large Volume Computation Clearing (LVCC). Neurons are labeled in green and astrocytes in red.

“Brains-In-A-Dish” from Induced Pluripotent Stem Cells (iPSCs)

This article discusses the benefits of using the THUNDER technology for imaging inside 3D human cortical brain organoids. These organoids are derived from human induced pluripotent stem cells (iPSCs)…
Spheroid shown here as a maximum projection of the raw widefield image data (left) and THUNDER image after Instant Computational Clearing (right). The images are derived from approximately 60 µm Z stacks. Different stains (alpha actin and vimentin) are used to help identify the various cell types. Images courtesy of Sandra Grijalva, Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, Atlanta, USA.

Developing Heart Pacemaker Cells from Cardiac Spheroids

During the last decade, 3D cell culture has been established as a more realistic model compared to classical 2D culture systems. Cells can develop into miniature 3D objects, so called spheroids, which…

将动态活细胞数据融入超微结构

采用徕卡Nano的工作流程,可以避免过去如海底捞针似的寻找。利用光电关联显微技术,在适当的时间直接鉴别出正确的细胞,并将动态的活细胞数据融入其超微结构中。
[Translate to chinese:] Electroporated nerve cells (green), specific neuronal markers (magenta) and cell nuclei (white), computational cleared.

利用 "Wow效应 "进入三维空间--以三维方式实时观察细胞

生命是瞬息万变的,对细胞来说更是如此。通常,细胞应在尽可能接近其自然环境的生理条件下进行检测。新技术为基于相机的荧光系统提供了巨大的性能,可在一次拍摄中实现全分辨率操作。本文介绍了如何利用新技术实时有效地去除焦平面以外区域不需要的图像内容。文章认为,这些新方法和数据交换正在推动科学进步。
[Translate to chinese:] Lung organoid taken at the "liquid-air interface" with a THUNDER Imager 3D Cell Culture. The cells originate from transgenic mice, so that the different fluorescence represents the degree of differentiation of the respective cell (superposition). The image acquisition was performed on day 21 after the start of the culture. Reference: P. Kanrai, MPI-HLR Bad Nauheim.

在发育过程中观察三维细胞培养

三维细胞培养物(如有机体和球形体)能让人们深入了解细胞及其与微环境的相互作用。这些三维细胞培养物在研究新型癌症疗法、治疗阿尔茨海默病的药物或研究癌症患者化疗效率的个性化微流体的研究人员中发挥着越来越重要的作用。有了新的成像系统,现在可以更容易地在细胞水平上实时检查三维细胞培养物的发育过程。对于三维样品的显微成像,有多种选择,如共聚焦显微镜或光片显微镜。
[Translate to chinese:] Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imager with a 2x 0.15 NA objective at 3.4x zoom was used to produce this 425 µm Z-stack (26 positions), which is presented here as an Extended Depth of Field (EDoF) projection.  Images courtesy of Dr. Fikri Birey  from the Dr. Sergiu Pasca laboratory at Stanford University, 3165 Porter Dr., Palo Alto, CA

下载活细胞成像指南

在生命科学研究中,活细胞成像是一种不可或缺的工具,可用于观察细胞的活体状态。这本电子书回顾了为确保成功进行活细胞成像而需要考虑的各种重要因素。
Dividing fission yeast S. pombe stained with two markers against spindle pole bodies (Pcp1-GFP, green) and cytokinesis ring (Rlc1-mCherry; red).

Studying Cell Division

Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
Images of the scaffold composed of fluorescent fibers: Left: raw widefield image. Right: THUNDER image with LVVC. Both images are maximal projections of a z stack of 55 images (total height of 130 µm). Images courtesy of Mollie Smoak, Department of Bioengineering, Rice University, Houston, TX, USA

Finding new Scaffolds for Tissue Engineering

Tissue engineers use biomaterials for a variety of applications from drug delivery to supporting the regeneration of damaged or lost tissues to creating in vitro disease models. Scaffold architecture…
[Translate to chinese:] Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA. Images courtesy of Dr. Reyna Martinez – De Luna, Upstate Medical University, Department of Ophthalmology.

自适应反卷积与 Computational Clearing 结合的力量

反卷积是一种计算方法,用于恢复被点扩散函数(PSF)和噪声源破坏的物体图像。在本技术简介中,您将了解徕卡显微系统提供的反卷积算法如何帮助您克服宽视场 (WF) 荧光显微镜中由于光的波动性和光学元件对光的衍射而造成的图像分辨率和对比度损失。探索由用户控制或自动反卷积的方法,查看并解析更多的结构细节。

Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics

Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell…
Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture. Image courtesy of Dr. Selina Keppler, Munich, Germany.

Image Gallery: THUNDER Imager

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…

From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy

Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…

Studying Human Brain Development and Disease

Neural spheroids created from human induced pluripotent stem cells (iPSCs) provide effective and novel tools for studying brain development, as well as the underlying pathological mechanisms of…
Mouse retina was fixed and stained by following reagents: anti-CD31 antibody (green): Endothelia cells, IsoB4 (red): Blood vessels, and microglia anti-GFAP antibody (blue): Astrocytes Sample courtesy by Jeremy Burton, PhD and Jiyeon Lee, PhD, Genentech Inc., South San Francisco, USA. Imaged by Olga Davydenko, PhD (Leica). Imaged with a THUNDER Imager 3D Cell Culture.

An Introduction to Computational Clearing

Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
Sep 24, 2020
故事
Factors to Consider When Selecting a Research Microscope

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
Influenca in lung epithelial cells (porcine) - THUNDER Imager 3D Cell Culture Influenca virus – red, cilia – green, Nuclei – blue.

How Can Immunofluorescence Aid Virology Research?

Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…

Computational Clearing - Enhance 3D Specimen Imaging

This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and improvements within a researcher's imaging-related…

THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows

This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in retina sections and RNA molecules in cancer tissue…

Evaluating Axon Regeneration After Brain or Spine Trauma of Mice

Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and…

Drosophila Testis Niche Stem Cells – Three Color Computational Clearing

Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only…

Alzheimer Plaques: fast Visualization in Thick Sections

More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…

Real Time Images of 3D Specimens with Sharp Contrast Free of Haze

THUNDER Imagers deliver in real time images of 3D specimens with sharp contrast, free of the haze or out-of-focus blur typical of widefield systems. They can even image clearly places deep inside a…

应用的领域

活细胞成像

将视角从单一的显微镜组件转向完整的活细胞成像解决方案,徕卡公司将显微镜、LAS X 成像软件、相机和第三方专用组件集成在一起,形成一个完整的活细胞成像系统。

荧光

荧光是生物和分析显微镜中最常用的物理现象之一,主要是因为它具有灵敏度高、特异性强的特点。荧光是冷发光的一种形式。用户可以通过显微镜来捕捉单个荧光分子的种类、分布、数量及其在细胞内的定位。用户可以进行荧光分子共定位和相互作用的研究,也可以观察在细胞内和细胞间运作离子浓度的变化,如胞吞和胞吐。借助超高分辨显微镜,我们甚至可以对亚细胞器的结构进行成像。

斑马鱼研究

为了在筛选、分拣、操作和成像过程中获取高质量结果,您需要观察细节和结构,从而为您的下一步研究做出正确的决策。 徕卡体视显微镜和透射光底座以出众的光学器件和优良的分辨率而闻名,是全世界研究学者的首选。

光操控

术语光操控包含一系列技术,具体是利用荧光分子的属性,启动细胞性活动,长时间观察活细胞中动态复合物的行为方式。不管是漂白、活化、转化、烧蚀,还是组合技术,研究员都需要一个能够完全执行和以高分辨率摄取细胞性活动的系统。

神经科学研究解决方案

您的工作是更好地了解神经退行性疾病,还是研究神经系统的功能? 了解如何使用徕卡显微系统的成像解决方案取得突破。

类器官和3D细胞培养

生命科学研究中最令人振奋的最新进展之一是3D细胞培养系统的发展,例如类器官、球状体或器官芯片模型。 3D细胞培养物是一种人工环境,在这种环境中,细胞能够在三维空间中生长并与周围环境相互作用。 这些环境条件与它们在体内的情况相似。

病毒学

您的主要研究对象是病毒感染和疾病吗? 了解如何使用徕卡显微系统公司的成像和样本制备解决方案深入研究病毒学。

先进显微镜技术

先进显微镜技术包括高分辨和超分辨成像技术。这些技术主要用于以极高的分辨率将生物事件可视化,同时对样本(通常是细胞或组织)尽可能地温和。 研究人员可以在先进显微镜技术的帮助下检查和理解对生物途径、基因或蛋白质表达、疾病机制等有重大影响的生物分子。

基础显微镜技术

使用基础显微镜技术,显微镜台上的整个标本将被暴露在光源下。整个标本被白光照亮,白光可以从上面(倒置光路配置)或下面(标准正置光路显微镜)照射。根据标本的透明度和感兴趣的目标或区域,采用不同的荧光粒子来区分感兴趣的复杂细胞结构。

DIC显微镜

DIC显微镜是一种宽视场显微镜,在光源镜与聚光镜之间,以及在物镜和相机传感器或目镜之间,都设有偏振滤光器和沃拉斯顿棱镜。

相差光学显微镜

使用相差光学显微镜,无需染色就可以更大对比度观察各种类型生物标本的结构。

暗场显微镜

此外,在对材料样本进行成像时,暗场显微镜还能增强图像对比度。暗场光学对比法利用生物标本结构或材料样本的不均匀特征产生的光散射或衍射。
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