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高压冷冻仪 Leica EM ICE

  • 病毒学

    您的主要研究对象是病毒感染和疾病吗? 了解如何使用徕卡显微系统公司的成像和样本制备解决方案深入研究病毒学。
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  • 模式生物研究

    模式生物是研究人员用来研究特定生物学过程的物种。 它们具有与人类相似的遗传特征,通常用于遗传学、发育生物学和神经科学等研究领域。 选择模式生物的原因通常是它们在实验室环境中易于保持和繁殖、生成周期短,或能够产生突变体来研究某些性状或疾病。
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  • The EM ICE Nano loading area
    Science Lab Topic: EM Sample Preparation

    How to Keep Your Samples Under Physiological Conditions

    Leica Microsystems’ Nano workflow combines dynamic data with the best possible sample fixation (EM ICE 70) by high pressure freezing. However, good sample preservation won’t help if your cells are…
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  • Science Lab Topic: EM Sample Preparation

    Capture Life as it Happens

    With the Leica Nano Workflow, searching for the needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…
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  • Science Lab Topic: EM Sample Preparation

    Putting Dynamic Live Cell Data into the Ultrastructural Context

    With the Leica Nano Workflow, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…
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  • Science Lab Topic: EM Sample Preparation

    Exploring the Structure and Life Cycle of Viruses

    The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…
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  • Science Lab Topic: EM Sample Preparation

    Fast, high-quality vitrification with the EM ICE High Pressure Freezer

    The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…
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  • Science Lab Topic: EM Sample Preparation

    Investigating Synapses in Brain Slices with Enhanced Functional Electron Microscopy

    A fundamental question of neuroscience is: what is the relationship between structural and functional properties of synapses? Over the last few decades, electrophysiology has shed light on synaptic…
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  • Science Lab Topic: EM Sample Preparation

    High-pressure freezing: Revealing functional mechanisms of synaptic transmission

    Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…
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  • Science Lab Topic: EM Sample Preparation

    Workflows and Instrumentation for Cryo-electron Microscopy

    Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
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  • Science Lab Topic: EM Sample Preparation

    Study synaptic dynamics with millisecond precision in neurobiology

    What if you could dissect the synaptic dynamics with millisecond precision? To resolve controversial questions in neurobiology employing new techniques for visualizing cellular activity is essential.…
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  • Science Lab Topic: EM Sample Preparation

    Ultrastructural Preservation and Improved Visualization of Membranes in Primary Bovine Chromaffin Cells

    Intracellular dynamic events, such as protein trafficking, recycling, and degradation as well as signal transduction, to name a few, can be studied using live-cell imaging techniques. However,…
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  • Roland A. Fleck
    Science Lab Topic: EM Sample Preparation

    Expert Knowledge on High Pressure Freezing and Freeze Fracturing in the Cryo SEM Workflow

    Get an insight in the working methods of the laboratory and learn about the advantages of Cryo SEM investigation in EM Sample Preparation. Find out how high pressure freezing, freeze fracturing and…
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  • Science Lab Topic: EM Sample Preparation

    Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation

    Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in…
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  • Science Lab Topic: EM Sample Preparation

    紫外线刺激防晒霜的Cryo-SEM分析

    EM ICE是唯一将光刺激与快速冷冻固定相结合的高压冷冻设备,也是神经生物学研究中使用最多的一种光遗传学方法。但这种方法对于制药或化妆品行业也非常值得研究,可用来深入了解UV(紫外线)照射对其目标结构的影响。本文举例说明UV(紫外线)照射对防晒霜超微结构的影响。
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  • Science Lab Topic: EM Sample Preparation

    Superior Ultrastructural Preservation and Structural Contrast in Drosophila Tissue

    Optimal structural preservation of tissue can only be obtained by high pressure freezing. However, preparing the samples in optimal conditions is challenging. This article explains in detail how to…
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  • Science Lab Topic: EM Sample Preparation

    Expanding the Limits of Electron Microscopy Sample Preparation

    Capturing the intricate changes in fine structure or in cell dynamics with conventional cryo solutions can be challenging sometimes. Leica Microsystems has developed a new cryo platform, the Leica EM…
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  • Science Lab Topic: EM Sample Preparation

    Revealing Cellular Dynamics with Millisecond Precision

    What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy?
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  • Science Lab Topic: EM Sample Preparation

    Interview with Dr. Shigeki Watanabe on Research in Synaptic Membrane Dynamics

    Dr. Shigeki Watanabe, principle investigator of the department of Cell Biology at the Johns Hopkins University School of Medicine in Baltimore, held a workshop in Zürich, Switzerland on methods to…
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  • Science Lab Topic: EM Sample Preparation

    Visualization of Membrane Dynamics with Millisecond Temporal Resolution

    Application Note for Leica EM ICE, Leica EM AFS2 - Electrical stimulation of neurons combined with high-pressure freezing allows physiological activation of synaptic activity and precise control over…
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  • Science Lab Topic: EM Sample Preparation

    Structural Study of C. elegans

    Application Note for Leica EM ICE, Leica EM AFS2 - Wildtype L4 stage C. elegans (N2 strain) were placed in the 100 μm deep side of Lecithin-coated (see detailed protocol*) type A 3 mm Cu/Au carriers…
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  • Science Lab Topic: EM Sample Preparation

    Cultured Rat Hippocampal Neurons

    Application Note for Leica EM ICE - Rat Hippocampal neurons, cultured on 50 μm thick Aclar (Aclar embedding film, EMS) for 19 days, were frozen in the 100 μm deep side of lecithin coated (detailed…
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  • Science Lab Topic: EM Sample Preparation

    Symmetric Synapse - Clathrin Coated Endocytosis Pit in the Postsynaptic Dendrite

    Application Note for Leica EM ICE - WT hippocampal neurons were plated at a density of 80,000 cells/cm2 on 6 mm sapphire disks for 14 days. Sample were frozen using a high-pressure freezer (Leica EM…
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  • Science Lab Topic: EM Sample Preparation

    High Pressure Freezing with Light Stimulation

    Sun screen lotion was carefully filled in the 100 μm incision of a 3 mm copper/gold plated flat carrier and covered with 3 mm sapphire disk. The sun screen lotion sample was then high pressure frozen…
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  • Science Lab Topic: CLEM

    CLEM: Combining the Strengths of Light and Electron Microscopy

    In recent years light microscopy studies have been dominated by live cell imaging while electron microscopy has been used for high-resolution studies. Latterly, there has been increasing interest in…
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