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06 May 2025
09 May 2025
Complete camera overview of EM grid recorded with 3 channels. Inserts displaying the positions, where superresolved 3D confocal images were recorded. 3D renderings of these positions are shown in the zoomed inserts. Fluorescence channels (nuclei by Hoechst, blue; mitochondria by MitoTracker Green, green; lipid Droplets by Bodipy and Crimson Beads, red). Width of a grid square is 90 ?m, width of a grid bar is 35 ?m. Samples kindly provided by Ievgeniia Zagoriy, Mahamid-Group, EMBL Heidelberg, Germany.

从显微镜到电镜:完整的冷冻光电联用工作流程

在题为“多模态玻璃化征程,从实验台到电子显微镜的冷冻关联工作流程”的网络研讨会上,专家团队(Edoardo D'Imprima、Zhengyi Yang、Andreia Pinto 和 Martin…

先进的细胞超微结构研究

冷冻断裂和冷冻蚀刻是研究柔性膜相关结构(如紧密连接或肠道糖萼)的有用工具。冷冻断裂和冷冻蚀刻是两种互补的方法,通过样品玻璃化来保护目标结构,然后断开冷冻标本以揭示内部结构。冷冻蚀刻是一个后续步骤,在真空下表面冰升华以揭示更多细节。在这些技术中,喷镀金属或碳使样品能够直接在冷冻扫描电子显微镜(SEM)中成像,或作为复制膜在透射电子显微镜(TEM)中成像。这对技术用于研究细胞器、膜、层和乳液,特别适用…

Workflows and Instrumentation for Cryo-electron Microscopy

Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
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