EM Cryo CLEM
电子显微镜样品制备
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- Front Microbiol. 2017 Apr 11;8:596; Pleomorphism and Viability of the Lyme Disease Pathogen Borrelia burgdorferi Exposed to Physiological Stress Conditions: A Correlative Cryo-Fluorescence and Cryo-Scanning Electron Microscopy Study; Vancová M, Rudenko N, Vaněček J, Golovchenko M, Strnad M, Rego ROM, Tichá L, Grubhoffer L, Nebesářová J.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387694/ - Nat Protoc. 2017 Jan;12(1):150-167; Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells; Hampton CM, Strauss JD, Ke Z, Dillard RS, Hammonds JE, Alonas E, Desai TM, Marin M, Storms RE, Leon F, Melikyan GB, Santangelo PJ, Spearman PW, Wright ER.
https://www.nature.com/articles/nprot.2016.168 - Methods Cell Biol. 2017;140:321-333; Matrix MAPS-an intuitive software to acquire, analyze, and annotate light microscopy data for CLEM; Schorb M, Sieckmann F.
http://www.sciencedirect.com/science/article/pii/S0091679X17300560 - Methods Cell Biol. 2017;140:303-320; triCLEM: Combining high-precision, room temperature CLEM with cryo-fluorescence microscopy to identify very rare events; Ader NR, Kukulski W.
http://www.sciencedirect.com/science/article/pii/S0091679X17300535 - Methods Cell Biol. 2017;140:85-103; A new method for cryo-sectioning cell monolayers using a correlative workflow; Kolovou A, Schorb M, Tarafder A, Sachse C, Schwab Y, Santarella-Mellwig R.
http://www.sciencedirect.com/science/article/pii/S0091679X17300559 - New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy tomography; Martin Schorb, Leander Gaechter, Ori Avinoam, Frank Sieckmann, Mairi Clarke, Cecilia Bebeacua, Yury S. Bykov, Andreas F.-P. Sonnen, Reinhard Lihl, John A. G. Briggs.
http://www.sciencedirect.com/science/article/pii/S1047847716301356 - Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions; Martin Strnad, Jana Elsterová, Jana Schrenková, Marie Vancová, Ryan O. M. Rego, Libor Grubhoffer & Jana Nebesárová.
https://www.nature.com/articles/srep18029 - Metskas LA, Briggs JAG - Fluorescence-Based Detection of Membrane Fusion State on a Cryo-EM Grid using Correlated Cryo-Fluorescence and Cryo-Electron Microscopy
Microscopy and Microanalysis. 2019 August; 25(4):942-949.
https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/fluorescencebased-detection-of-membrane-fusion-state-on-a-cryoem-grid-using-correlated-cryofluorescence-and-cryoelectron-microscopy/4933BFD9777A1E735075BFE5A60DFC90#fndtn-information
