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Leica EM ACE600 溅射镀膜机

碳、电子束和溅射镀膜——精度值得信赖

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Exploring the Structure and Life Cycle of Viruses

The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…

Workflows and Instrumentation for Cryo-electron Microscopy

Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…

研究天然聚合物精细细节的微观结构

本报告评估了结合使用冷冻宽幅离子束铣削和扫描电子显微镜(cryo-BIB-SEM)对低温稳定柔性聚合物的微观结构进行成像和分析的潜能。报告介绍了使用cryo-BIB-SEM对易损天然聚合物进行检查的结果,例如番茄果皮和木材,还分析了聚合物表面形态和多种微观结构特性。

Macroscale to Nanoscale Pore Analysis of Shale and Carbonate Rocks

Physical porosity in rocks, like shale and carbonate, has a large effect on the their storage capacity. The pore geometries also affect their permeability. Imaging the visible pore space provides…

Visualization of DNA Molecules

Precise low angle rotary shadowing with heavy metals (like platinum) can be used in transmission electron microscopy (TEM) to observe molecular details of objects previously absorbed on a thin, low…

Improvement of Metallic Thin Films for HR-SEM by Using DC Magnetron Sputter Coater

Preparation techniques, like several kinds of coating methods play an important role for high resolution scanning electron microscopy (HR-SEM). Nonconductive sample like biological and synthetic…

冷冻断裂与冷冻蚀刻基础介绍

冷冻断裂是一种将冰冻样本劈裂以露出其内部结构的技术。冷冻蚀刻是指让样本表面的冰在真空中升华,以便露出原本无法观察到的断裂面细节。金属/碳复合镀膜能够实现样本在SEM(块面)或TEM(复型)中的成像,主要用于研究如细胞器、细胞膜,细胞层和乳胶。这项技术传统上用于生物学应用,但现在逐渐在物理学和材料科学中展现出重要意义。近年来,研究人员通过冷冻断裂电子显微镜,尤其是冷冻复型免疫标记(FRIL),对膜蛋…

Glycerol Spraying/Platinum Low Angle Rotary Shadowing of DNA with the Leica EM ACE600 e-beam

Glycerol spraying/low angle rotary shadowing (Aebi and Baschong, 2006) is a preparation technique used in biology to visualize structures yielding insufficent contrast with other techniques, due to…

电子显微镜镀膜技术简要介绍

电子显微镜领域需要对样本进行镀膜处理才能改善样本的成像效果。在样本上形成一层金属导电层可抑制电荷聚积、减少热损伤,并改善SEM对样品拓扑结构检测所需的二次电子信号量。在x射线显微分析中,网格上支持膜,TEM观察复型样品中的背底支撑膜,涉及既对电子束透明但同时具备导电效果的精细碳膜。具体需要采用的镀膜技术取决于分辨率和应用。

电子显微镜碳厚度

在电子显微镜样品镀膜时,石英晶振片可控制镀膜的厚度。这些晶振片一定的频率(新晶体时约为6兆赫)进行振荡。通过测量镀膜前和镀膜后的频率、镀膜材质比重和石英的几何位置,可以计算出应用厚度。

应用的领域

汽车 & 交通工业显微镜

徕卡希望成为您密不可分的合作伙伴,带给您成像解决方案,让您在竞争中保持前列地位。 徕卡智能成像解决方案可助您应对特殊应用,满足当前和未来的需要,让您专心致志进行创新。 …

工业显微镜市场

最大限度地提高正常运行时间并有效地实现目标有助于您提升效益。徕卡显微镜解决方案可以让您深入了解微小)的样品细节,以及快速可靠地分析,记录和报告结果。徕卡微系统提供广泛的解决方案和专家支持,可以帮助您满足不同的应用需求。
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