ATTO 655
ATTO 655 belongs together with ATTO 680 and ATTO 700 to a new generation of 荧光标记物s.
Characteristic features of the label are strong absorption, high 荧光量子产率, high thermal and photo-stability, exceptionally high stability towards atmospheric ozone, and very good water solubility. Thus ATTO 655 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.
ATTO 655 是一种zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 655 是一种strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. The fluorescence is excited most efficiently in the range 640 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
