Leica Microsystems
Leica Microsystems is a world leader in microscopes and scientific instruments. Founded as a family business in the nineteenth century, the company’s history was marked by unparalleled innovation on its way to becoming a global enterprise.
Its historically close cooperation with the scientific community is the key to Leica Microsystems’ tradition of innovation, which draws on users’ ideas and creates solutions tailored to their requirements. At the global level, Leica Microsystems is organized in three divisions, all of which are among the leaders in their respective fields: Life Science, Industry and Medical.
The company is represented in over 100 countries with 6 manufacturing facilities in 5 countries, sales and service organizations in 20 countries, and an international network of dealers. The company is headquartered in Wetzlar, Germany.
AI-Based Particle Counting with the Aivaro AI Feature Detection Software Module
The Aivaro AI Feature Detection software module enables fast manual microscope inspection of particulate contamination on various material samples. By training different classes, reflective particles,…
AI Image Analysis for Reliable Defect Detection
AI-based visual inspection with microscopy helps make quality control in industrial environments efficient by reducing manual inspection effort concerning defect detection, classification, and…
An Overview of Artificial Intelligence (AI) and Machine Learning
AI is rapidly changing industrial inspection, quality control, and manufacturing workflows. But not all AI technologies work the same way. From machine learning and deep learning to the newer concept…
Why the Future of Microscopic Inspection Is AI-Assisted, Not Fully Automated
The next wave of QC productivity gains will not come from building more automated inspection lines. Instead, it will come from augmenting millions of inspection decisions that are made manually every…
Fluorescence-Guided Cryo-ET of Malaria Parasites
Correlative cryo workflow with cryo-planing and cryo-fluorescence imaging enables targeted cryo-ET of parasite-rich regions in complex tissue.
Cryo-ET Sample Preparation: From Waffle Method to Serial Lift-Out
Cryo-ET sample preparation becomes more demanding when specimens are thicker, larger, or more complex. This webinar brings together four perspectives on how high-pressure freezing can be connected…
如何深入了解类器官和细胞球模型
在本电子书中，您将了解3D细胞培养模型（如类器官和细胞球）成像的关键注意事项。探索创新型显微镜解决方案，来实时记录类器官和细胞球的动态成像过程。
Waffle Method Workflow: From HPF to Cryo-ET Lamellae
Waffle freezing provides an HPF-based route to cryo-ET sample preparation. This workflow guide follows the process from grid and carrier assembly to vitrification, cryo-FIB milling, lamella…
Fast, High-Contrast Widefield Imaging of Optically Challenging Samples
Live‑cell imaging of large, complex biological samples often requires large fields of view, sub-cellular resolution, high-sensitivity, and fast acquisition – all while maintaining low illumination…
Dental Loupes vs Microscopes: Exploring Visualization Options in Dentistry
Dental professionals often ask: “Should I use dental loupes or invest in a microscope?” This article explores the key differences between dental microscopes and dental loupes, focusing on…
Spatial Proteomics Workflow in Blood Cancer (MPNs)
Megakaryocytes play a central role in the biology of myeloproliferative neoplasms (MPNs), yet their in vivo proteomic characterization remains a major challenge due to low abundance and disrupted…
选择牙科显微镜时需考虑的六大特性
在牙医学中，手术显微镜对于进行高质量和成功的手术来说变得愈发重要，尤其是在牙髓病学领域。显微镜协助牙医进行微创手术，旨在保护牙质、保留组织、最大限度地降低风险并缩短愈合时间。
要选择适合牙医需求的显微镜，了解现代牙科显微镜的一些决定性特征将十分有帮助。
Multiscale Imaging of Organoids: High Content to Light Sheet
Learn multiscale organoid imaging: fixed high content phenotyping, gentle dual view light sheet, and reproducible pipelines that turn 3D data into insights.
比例成像
细胞的许多基本功能在很大程度上依赖于离子（例如钙、镁）、电压势和细胞质与周围细胞外空间之间的 pH 值的微妙但动态的平衡。这些平衡的变化会显著改变细胞的行为和功能。因此，实时测量细胞内离子、电压和 pH…
荧光寿命成像与荧光共振能量转移
荧光寿命是荧光团在发射荧光光子返回基态之前保持其激发态的平均时间长度。这取决于荧光团的分子组成和纳米环境。
FLIM将寿命测量与成像相结合：对每个图像像素以测得的荧光寿命进行颜色编码，产生额外的图像反差。因此，FLIM可以提供关于荧光分子空间分布的信息和有关其生化状态或纳米环境的信息。…
4 Key Benefits of 3D Digital Microscopy in Ophthalmic Surgery
3D digital visualization is rapidly transforming ophthalmic surgery. Modern 3D surgical microscopes enable surgeons to perform procedures using high-resolution digital displays rather than traditional…
High-Pressure Freezing Protocols for Ultrastructural 3D EM
High pressure freezing (HPF) can help preserve hydrated cells and tissues close to their biological state at the moment of immobilization, supporting more reliable ultrastructural interpretation than…
Ultramicrotome UC Enuity in Practice: Stable 15 nm Sections at ZFE
After using the UCT and UC6 ultramicrotomes, Claudia Mayrhofer calls UC Enuity a leap in stability—so robust that vibrations and temperature shifts don’t spoil sections, even with multiple users. Auto…
利用偏光显微镜优势确保玻璃质量
玻璃是已知最古老的材料之一。如今，玻璃被广泛应用于各种领域，如光学仪器、门窗、太阳能电池板、食品、饮料和药品容器等，因此必须符合严格的玻璃质量标准，尤其是光学玻璃。利用偏光显微镜对平板玻璃、中空玻璃和压制玻璃进行质量控制既快捷又经济。无需进行耗时的样品制备，即可对结节、金属、晶体夹杂物和气泡等缺陷进行分析。
Expert Techniques for Superior Visualization in Cataract Surgery
Join renowned ophthalmic surgeons, Dr. Hussein Almuhtaseb and Mr. Simon Madge, as they share their clinical expertise and real-world surgical strategies during the 2025 Online Cataract Surgery…
癌症研究中显微镜的历史、发展和趋势
癌症是一种全球性疾病，2020 年全球将新增 1800 万确诊病例，1000 万人死于癌症。预计到 2040 年，病例数将增加约 55%。研究了解癌症的发生、发展以及开发新的诊断工具和治疗方法至关重要。
荧光染料应用和特性概述
本文将介绍常用的荧光染料并概述其特性。荧光显微镜借助荧光染料、荧光蛋白或使用抗体的免疫荧光染色来研究特定的细胞成分。由于荧光剂种类繁多，荧光显微镜可用于检查蛋白质、核酸、聚糖、细胞器和其他细胞结构。
Researchers Insights: Microscopy in Cancer Research
Discover how imaging techniques are driving cancer research forward. In this issue, we present comprehensive multimodal studies using microscopy, as well as new directions in intraoperative cancer…
Predictive Service Prevents Downtime in Ghent
At the VIB BioImaging Core in Ghent, Belgium, researchers depend on Leica’s Stellaris 8 confocal microscope to explore the frontiers of biomedical science. When Leica’s RemoteCare system detected a…
显微镜中的荧光
荧光显微技术是一种特殊的光学显微镜技术。它利用的是荧光色素在一定波长的光激发下发光的能力。通过抗体染色或荧光蛋白标记，可以用这种荧光色素标记感兴趣的蛋白质。这样就可以确定单分子物种的分布、数量及其在细胞内的定位。此外，还可以进行共定位和相互作用研究，使用可逆结合染料（如 Ca2+ 和 fura-2）观察离子浓度，以及观察细胞的内吞和外吞过程。如今，利用荧光显微镜甚至可以对亚分辨率颗粒进行成像。
如何选择合适的测量显微镜
使用测量显微镜，用户可以测量样品特征的二维和三维尺寸，这对检测、质量控制、故障分析和研发&D 至关重要。然而，选择合适的显微镜需要评估应用需求以及显微镜的性能、易用性和灵活性。 如今，测量通常以数字方式进行，即使用带有摄像头和软件的显微镜，图像显示在显示器上，而不是通过目镜网线，从而提高了精度和可重复性。使用合适的测量显微镜可靠、快速地分析样品。
显微镜测量校准：为什么要这样做？
显微镜校准可确保检测、质量控制 (QC)、故障分析和研发 (R&D) 所需的测量准确一致。本文介绍了校准步骤。使用参照物进行校准可使结果具有可重复性，并有助于确保与准则和标准一致。为了获得准确一致的结果，建议校准显微镜并定期检查。如有需要，可向校准专家寻求支持。
晶圆表面光刻胶残留与有机污染物可视化检测
随着半导体集成电路 (IC) 的尺寸缩小到 10 纳米以下，在晶圆检测过程中有效检测光刻胶残留物等有机污染物和缺陷变得越来越重要。光学显微镜仍是常用的检测方法，但对于有机污染而言，明视野和其他类型的照明都有其局限性。本文讨论了在半导体行业的质量控制、故障分析和研发&D 过程中，如何利用荧光显微镜有效检测晶片上的光刻胶残留物和其他有机污染物。
Rapidly Visualizing Magnetic Domains in Steel with Kerr Microscopy
The rotation of polarized light after interaction with magnetic domains in a material, known as the Kerr effect, enables the investigation of magnetized samples with Kerr microscopy. It allows rapid…
6 英寸晶片检测显微镜，可靠的观察微小高度差
本文介绍了一种 6 英寸晶圆检测显微镜，无论用户的技术水平如何，它都能自动进行可重复的 DIC（微分干涉对比）成像。集成电路 (IC) 芯片和半导体元件的制造需要晶圆检测，以确保不存在影响性能的缺陷。通常使用光学显微镜进行质量控制、故障分析和 R&D 检测。为了有效地观察晶圆上结构之间的微小高度差，可以使用 DIC。
