共聚焦显微镜针孔效应
在操作共聚焦显微镜,或在讨论这种装置的特性和参数时,我们不可避免地提到针孔及其直径。这篇简短的文章是针对那些没有足够时间钻研共聚焦显微镜的理论和细节但又想了解针孔效应的用户们来解释针孔的意义。
Rodent and Small-Animal Surgery
Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.
Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
显微镜下的慢性炎症
在慢性炎症的过程中,身体的某些部位会反复发炎。许多人类疾病都是如此。在宽场光学显微镜的帮助下,可以对从细胞水平到整个生物体的潜在过程进行检查。本文介绍了几种宽场显微镜应用,如免疫荧光、活细胞成像、组织学和比率分析,以深入了解慢性炎症的发展、相关疾病及其治疗。
Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering
The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target…
Imaging and Analyzing Zebrafish, Medaka, and Xenopus
Discover how to image and analyze zebrafish, medaka, and Xenopus frog model organisms efficiently with a microscope for developmental biology applications from this article.
细菌实验方案 - 对大肠杆菌样本实施临界点干燥以进行SEM分析
徕卡EM CPD300的应用文档 - 生命科学研究,对大肠杆菌样本实施临界点干燥后,进行铂/钯金属镀膜,后用SEM拍摄微观形貌,将样本放入一个滤盘中(孔径:16-40μm),置入滤盘和多孔样品架内。在含有培养基的琼脂上培养真菌和细菌,为期3天。选取部分细菌菌落。
研究果蝇(黑腹果蝇Drosophila melanogaster)
由于每个实验室的需求可能会有很大的差异,本文展示了科学家和技术人员研究果蝇并使用不同显微镜设置的的实例。此外,基于不同果蝇实验室的经验介绍了推荐的工作流程。本文可以作为建立或扩展果蝇实验室时的参考或指南。