高压冷冻技术:揭示突触传递的功能机制

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深入了解如何在 EM ICE 中应用光遗传学刺激技术,以及该技术如何有望揭示突触传递的结构与功能机制。获取关于如何将光遗传学刺激应用于小鼠急性脑切片和器官型脑切片培养物中完整神经网络的详细介绍。

核心学习要点

  • Preparing brain tissue (acute slices and organotypic slice cultures) with high-pressure freezing.
  • Strategies to optimize the freezing of brain tissue.
  • Strategies to optimize the light stimulation of brain tissue prepared with high-pressure freezing.
  • Optimized freeze substitution for brain tissue specimens after light stimulation.
  • Possible future applications for brain tissue specimens prepared with high-pressure freezing and observed with light-stimulation.
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