THUNDER Imager Tissue全景组织显微成像系统
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THUNDER Imager Tissue全景组织显微成像系统
实时解构3D生物微观世界*
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![[Translate to chinese:] Brain organoid section (DAPI) acquired using THUNDER Imager Live Cell. Image courtesy of Janina Kaspar and Irene Santisteban, Schäfer Lab, TUM.](/fileadmin/_processed_/2/7/csm_Tilescan_of_brain_organoid_section_46d510ba4e.jpg "[Translate to chinese:] Brain organoid section (DAPI) acquired using THUNDER Imager Live Cell. Image courtesy of Janina Kaspar and Irene Santisteban, Schäfer Lab, TUM.")
研究大脑健康的成像类器官模型
小胶质细胞是特化的脑驻留免疫细胞，在大脑发育、平衡和疾病中发挥着至关重要的作用。然而，到目前为止，模拟人脑环境与小胶质细胞之间相互作用的能力还非常有限。
![[Translate to chinese:] Mouse cortical neurons. Transgenic GFP (green). Image courtesy of Prof. Hui Guo, School of Life Sciences, Central South University, China](/fileadmin/_processed_/2/a/csm_THUNDER_Imager_Mouse_cortical_neuron_9d4054774d.jpg "[Translate to chinese:] Mouse cortical neurons. Transgenic GFP (green). Image courtesy of Prof. Hui Guo, School of Life Sciences, Central South University, China")
显微镜如何帮助研究机械感受和突触通路
Tobi Langenhan教授使用显微镜研究突触蛋白质组合体，研究粘附性GPCR的机械感受特性，并了解蛋白质动力学及其空间相互作用。
What are the Challenges in Neuroscience Microscopy?
eBook outlining the visualization of the nervous system using different types of microscopy techniques and methods to address questions in neuroscience.
Going Beyond Deconvolution
Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen…
快速、高灵敏度成像和人工智能辅助分析
The specificity of fluorescence microscopy allows researchers to accurately observe and analyze biological processes and structures quickly and easily, even when using thick or large samples. However,…
为活细胞成像创造新选择
对厚实的活体样本进行成像时，主要挑战之一是获得图像质量与组织完整性之间的平衡。长时间的图像采集期间，弱信号光会导致低信号水平，导致图像对比度低以及分割和分析困难。需要通过高剂量成像或高时间分辨率成像技术加强信号强度时，这一问题更加突出。一个常见问题是：我如果快速成像、一次完成，会不会造成样本过度漂白或者细胞死亡？
感觉神经元的高对比度快速三维成像
本文讨论了相比传统的宽场显微镜，使用large volume computational clearing（LVCC）技术的THUNDER组织成像系统如何获取背根神经节（DRG）组织高对比度的快速三维成像图，获得感觉神经元更为清晰的解析图像。神经科学研究的一项主要领域集中在感觉神经元对触觉和痛觉的影响方面。深入理解这种现象对于神经系统疾病和疗法的发展具有重要意义。
精确分析宽视野荧光图像
利用荧光显微镜的特异性，即便是使用厚样品和大尺寸样品，研究人员也能够快速轻松地准确观察和分析生物学过程和结构。然而，离焦荧光会提高背景荧光，降低对比度，影响图像的精确分割。THUNDER 与Aivia 的组合可以有效解决这一问题。前者可以消除图像模糊，后者会使用人工智能技术自动分析宽视野图像，提高操作速度和精确性。下面，我们来详细了解下这一协作方法。
![[Translate to chinese:] Raw widefield (left) and THUNDER (right) image of Ewing Sarcoma cells (SK-ES-1).](/fileadmin/academy/2022/Visualizing_the_Mitotic_Spindle_in_Cancer_Cells/Tubulin_mitotic_spindle_teaser.jpg "[Translate to chinese:] Raw widefield (left) and THUNDER (right) image of Ewing Sarcoma cells (SK-ES-1)")
肿瘤细胞中有丝分裂纺锤体的成像
本文展示了如何使用THUNDER Imager Tissue和Large Volume Computational…
High-resolution 3D Imaging to Investigate Tissue Ageing
Award-winning researcher Dr. Anjali Kusumbe demonstrates age-related changes in vascular microenvironments through single-cell resolution 3D imaging of young and aged organs.
Optimizing THUNDER Platform for High-Content Slide Scanning
With rising demand for full-tissue imaging and the need for FL signal quantitation in diverse biological specimens, the limits on HC imaging technology are tested, while user trainability and…
Physiology Image Gallery
Physiology is about the processes and functions within a living organism. Research in physiology focuses on the activities and functions of an organism’s organs, tissues, or cells, including the…
Developmental Biology Image Gallery
Developmental biology explores the development of complex organisms from the embryo to adulthood to understand in detail the origins of disease. This category of the gallery shows images about…
![[Translate to chinese:] Electroporated nerve cells (green), specific neuronal markers (magenta) and cell nuclei (white), computational cleared.](/fileadmin/_processed_/a/0/csm_Ferret_Brain_LVCC_30911a5848.jpg "[Translate to chinese:] Electroporated nerve cells (green), specific neuronal markers (magenta) and cell nuclei (white), computational cleared.")
利用 "Wow效应 "进入三维空间--以三维方式实时观察细胞
生命是瞬息万变的，对细胞来说更是如此。通常，细胞应在尽可能接近其自然环境的生理条件下进行检测。新技术为基于相机的荧光系统提供了巨大的性能，可在一次拍摄中实现全分辨率操作。本文介绍了如何利用新技术实时有效地去除焦平面以外区域不需要的图像内容。文章认为，这些新方法和数据交换正在推动科学进步。
Studying Cell Division
Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
![[Translate to chinese:] Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA. Images courtesy of Dr. Reyna Martinez – De Luna, Upstate Medical University, Department of Ophthalmology.](/fileadmin/_processed_/3/a/csm_The_Power_of_Pairing_Adaptive_Deconvolution_teaser_5d4bdbe29b.jpg "[Translate to chinese:] Image: Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA.")
自适应反卷积与 Computational Clearing 结合的力量
反卷积是一种计算方法，用于恢复被点扩散函数（PSF）和噪声源破坏的物体图像。在本技术简介中，您将了解徕卡显微系统提供的反卷积算法如何帮助您克服宽视场 (WF) 荧光显微镜中由于光的波动性和光学元件对光的衍射而造成的图像分辨率和对比度损失。探索由用户控制或自动反卷积的方法，查看并解析更多的结构细节。
Improvement of Imaging Techniques to Understand Organelle Membrane Cell Dynamics
Understanding cell functions in normal and tumorous tissue is a key factor in advancing research of potential treatment strategies and understanding why some treatments might fail. Single-cell…
Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…
Studying Human Brain Development and Disease
Neural spheroids created from human induced pluripotent stem cells (iPSCs) provide effective and novel tools for studying brain development, as well as the underlying pathological mechanisms of…
An Introduction to Computational Clearing
Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
Computational Clearing - Enhance 3D Specimen Imaging
This webinar is designed to clarify crucial specifications that contribute to THUNDER Imagers' transformative visualization of 3D samples and improvements within a researcher's imaging-related…
THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows
This webinar will showcase the versatility and performance of THUNDER Imagers in many different life science applications: from counting nuclei in retina sections and RNA molecules in cancer tissue…
Evaluating Axon Regeneration After Brain or Spine Trauma of Mice
Damaged nerve regeneration was investigated using mouse spinal cord sections treated with compounds that counter axon growth inhibitor (AGI) proteins. The sections were screened to find active and…
Drosophila Testis Niche Stem Cells – Three Color Computational Clearing
Differentiated living beings such as humans, but also a fruit fly or a plant, possess not only the differentiated cells which form specific tissues, but also those cells whose fate is not yet (or only…
Alzheimer Plaques: fast Visualization in Thick Sections
More than 60% of all diagnosed cases of dementia are attributed to Alzheimer’s disease. Typical of this disease are histological alterations in the brain tissue. So far, there is no cure for this…
Real Time Images of 3D Specimens with Sharp Contrast Free of Haze
THUNDER Imagers deliver in real time images of 3D specimens with sharp contrast, free of the haze or out-of-focus blur typical of widefield systems. They can even image clearly places deep inside a…
应用的领域
荧光
荧光是生物和分析显微镜中最常用的物理现象之一，主要是因为它具有灵敏度高、特异性强的特点。荧光是冷发光的一种形式。用户可以通过显微镜来捕捉单个荧光分子的种类、分布、数量及其在细胞内的定位。用户可以进行荧光分子共定位和相互作用的研究，也可以观察在细胞内和细胞间运作离子浓度的变化，如胞吞和胞吐。借助超高分辨显微镜，我们甚至可以对亚细胞器的结构进行成像。
神经科学研究解决方案
您的工作是更好地了解神经退行性疾病，还是研究神经系统的功能？ 了解如何使用徕卡显微系统的成像解决方案取得突破。
斑马鱼研究
为了在筛选、分拣、操作和成像过程中获取高质量结果，您需要观察细节和结构，从而为您的下一步研究做出正确的决策。
徕卡体视显微镜和透射光底座以出众的光学器件和优良的分辨率而闻名，是全世界研究学者的首选。
活细胞成像
将视角从单一的显微镜组件转向完整的活细胞成像解决方案，徕卡公司将显微镜、LAS X 成像软件、相机和第三方专用组件集成在一起，形成一个完整的活细胞成像系统。
癌症研究
癌症是一种复杂的异质性疾病，由于细胞生长失控而引起。 一个或一组细胞的基因和表观遗传的变化破坏了正常功能，导致细胞自发、不受控制地生长和增殖。
病毒学
您的主要研究对象是病毒感染和疾病吗？ 了解如何使用徕卡显微系统公司的成像和样本制备解决方案深入研究病毒学。
DIC显微镜
DIC显微镜是一种宽视场显微镜，在光源镜与聚光镜之间，以及在物镜和相机传感器或目镜之间，都设有偏振滤光器和沃拉斯顿棱镜。
相差光学显微镜
使用相差光学显微镜，无需染色就可以更大对比度观察各种类型生物标本的结构。
暗场显微镜
此外，在对材料样本进行成像时，暗场显微镜还能增强图像对比度。暗场光学对比法利用生物标本结构或材料样本的不均匀特征产生的光散射或衍射。
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