THUNDER Imager Cell Spinning Disk系统

通过协同作用提高清晰度

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Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imager with a 2x, 0.15 NA objective at 3.4x zoom was used to produce this 425 µm Z-stack (26 positions) which is presented here as an Extended Depth of Field (EDoF) projection. Images courtesy of Dr. F. Birey, Dr. S. Pasca laboratory, Palo Alto, CA.

Guide to Live-Cell Imaging

For a wide range of applications in various research fields of life science, live-cell imaging is an indispensable tool for visualizing cells in a state as close to in vivo, i.e. living and active, as…
Shown is the DMi8 inverted microscope which is used for life-science research.

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
Brain organoid labeled with lamin (green) and tubulin (magenta), acquired using Viventis Deep. Courtesy of Akanksha Jain, Treutlein Lab ETH-DBSSE Basel (Switzerland).

如何深入了解类器官和细胞球模型

在本电子书中,您将了解3D细胞培养模型(如类器官和细胞球)成像的关键注意事项。探索创新型显微镜解决方案,来实时记录类器官和细胞球的动态成像过程。
Image of roundworm C. elegans acquired with a M205 FA fluorescence automated stereo microscope in combination with Rottermann contrast. Areas labelled with mCherry are seen as reddish purple.

线虫研究指南 - 针对线虫的相关工作

本指南概述了可以高效进行线虫的研究显微镜技术。线虫是一种广泛使用的模式生物,与人类有大约 70% 的基因同源性,是研究发育、神经科学、遗传学和衰老的理想生物。它的透明性和易培育性使其成为一个出色的遗传学模型系统。它可以进行高分辨率成像。主要的实验方法包括挑虫、转基因、荧光筛选、成像和记录。
Boston Innovation Hub

Boston and San Francisco Innovation Hubs

Boston and San Francisco Innovation Hubs are here to help you advance scientific discovery. We provide researchers access to state-of-the-art microscope technology and expert guidance. Located in the…
Image: Human stem cell-derived mid brain organoids. Courtesy of Dr Tanya Singh, University of Oxford.

揭开类器官模型在生物医学研究中的秘密

准备深入了解类器官和3D培养物的世界,它们是促进我们了解人类健康的重要工具。浏览这些复杂的结构并获取清晰的图像进行分析是一项挑战。在本次活动中,来自牛津大学和伦敦大学学院的研究人员将与我们一起展示Thunder Imager Cell转盘共聚焦系统 如何提供更有说服力的高质量数据,以便深入了解各种模型。
微管蛋白的 TIRF 图像,YFP 标记,穿透深度:120 毫米

全内反射荧光显微镜(total internal reflection fluorescent microscope,TIRFM)在生命科学研究中的应用

全内反射荧光显微镜的独特之处在于利用衰逝波激发荧光团。与传统的弧光灯、LED 或激光宽场荧光照明方式不同,衰逝波仅能从盖玻片/介质界面开始穿透样本约 100 纳米深度。
表达 GFP 标记的细胞粘附分子 CD44 的乳腺癌肿瘤细胞的 TIRF 图像,该分子位于细胞膜上,通过 TIRF 成像。

全内反射荧光(total internal reflection fluorescent microscope,TIRF)显微镜

全内反射荧光(TIRF)是荧光显微镜技术中的一项特殊技术,由密歇根大学安娜堡分校的 Daniel Axelrod 于 1980 年代初开发。TIRF 显微镜能提供轴向分辨率低于 100 纳米的超高清晰图像,这使得观察膜相关过程成为可能。
Murine esophageal organoids (DAPI, Integrin26-AF 488, SOX2-AF568) imaged with THUNDER Imager Cell. Courtesy of Dr. F.T. Arroso Martins, Tamere University, Finland.

Biopharma

For biopharma, Leica solutions help accelerate drug discovery, enhance cellular analysis, and support data integrity that meets regulations.

应用领域

类器官和3D细胞培养

生命科学研究中最令人振奋的最新进展之一是3D细胞培养系统的发展,例如类器官、球状体或器官芯片模型。 3D细胞培养物是一种人工环境,在这种环境中,细胞能够在三维空间中生长并与周围环境相互作用。 这些环境条件与它们在体内的情况相似。
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