David Pointu , Ph.D.
David Pointu studied physical chemistry at the University of Strasbourg. After completing his PhD in 2002, and a postdoctoral fellowship at the Institut Curie, he moved to pursue a career in industry in 2004. He has since worked as an application specialist in high-end microscopy at different companies including GE Healthcare. In 2021 he joined Leica Microsystems as an application manager for Cell DIVE and translational research. David has broad experience in light microscopy, including various methods, samples and workflow for cell and tissue imaging and analysis.
![[Translate to chinese:] Co-detection of 10 extracellular matrix proteins and 3 topographical tissue landmarks by multiplex immunostaining within a single high-grade fibrous hotspot from a human hepatocellular carcinoma](/fileadmin/_processed_/0/8/csm_Single_high-grade_fibrous_hotspot_from_a_human_hepatocellular_carcinoma_ec79be2bf1.jpg "[Translate to chinese:] Co-detection of 10 extracellular matrix proteins and 3 topographical tissue landmarks by multiplex immunostaining within a single high-grade fibrous hotspot from a human hepatocellular carcinoma")
肝细胞癌中癌症干细胞位点的原位鉴定
在这里，我们探索了一种突破性的多重免疫检测方法，通过多重成像对细胞外基质（ECM）特征进行原位定位，从而识别肝细胞癌（HCC）内的癌症干细胞龛。
利用Cell DIVE 在单细胞水平上进行超复杂癌症组织分析
能够研究淋巴瘤细胞的异质性如何受到细胞对其微环境反应的影响，尤其是在突变、转录组和蛋白质水平上。蛋白质表达研究提供了有关细胞相互作用性质和蛋白质表达水平的最相关信息。超复合工作流程可用于研究同一癌症组织中的多种蛋白质。
