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DMi8 S 模块 INFINITY TIRF 全内反射荧光系统

  • [Translate to chinese:]

    活细胞成像

    将视角从单一的显微镜组件转向完整的活细胞成像解决方案,徕卡公司将显微镜、LAS X 成像软件、相机和第三方专用组件集成在一起,形成一个完整的活细胞成像系统。
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  • 徕卡照相术

    光操控

    术语光操控包含一系列技术,具体是利用荧光分子的属性,启动细胞性活动,长时间观察活细胞中动态复合物的行为方式。不管是漂白、活化、转化、烧蚀,还是组合技术,研究员都需要一个能够完全执行和以高分辨率摄取细胞性活动的系统。
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  • [Translate to chinese:] Leica Cell Biology

    细胞生物学

    如果您的研究重点是探究人类健康和疾病相关的细胞学基础,那么从时空和分子层面详细研究感兴趣的细胞至关重要。 因此,显微成像是细胞生物学中一个非常重要的工具,它让您能够在样本的结构环境中详细研究样本,也可以分析细胞器和大分子。 细胞生物学成像是运用一系列的光学显微镜和电子显微镜完成的。 徕卡显微系统公司推出的成像解决方案专为扩展您的细胞生物学研究而设计。
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  • 病毒学

    您的主要研究对象是病毒感染和疾病吗? 了解如何使用徕卡显微系统公司的成像和样本制备解决方案深入研究病毒学。
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  • Images from 2 time-lapse series of the same Sf21 moth larval cells
    Science Lab Topic: Widefield Microscopy

    Studying Actin Cytoskeleton and Microfilament Assembly

    This article demonstrates how actin microfilament assemblies can be clearly visualized with superior resolution at the plasma membrane of a cell using a DMi8 S Infinity TIRF High Power imaging…
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  • Science Lab Topic: Fluorescence Microscopy

    Plant Cell Development and Morphogenesis

    The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…
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  • Science Lab Topic: TIRF Microscopy

    TIRF Publication List

    This monthly updated references list presents current papers using Leica AM TIRF in the major application fields for TIRF microscopy.
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  • Science Lab Topic: Live-Cell Imaging

    Where to go? Cellular Migration requires coordinated Transitions of Actin Cortex

    Plants, Bacteria, and Fungi possess a rigid cell wall that protects the cell and gives it shape. Animal cells, such as mammalian cells, have no outer wall, which exposes their plasma membrane to the…
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  • Science Lab Topic: Super-Resolution

    The Molecular Architecture of Hemidesmosomes as Revealed by Super-Resolution Microscopy

    Hemidesmosomes have been extensively studied by immunofluorescence microscopy, but due to its limited resolution, their precise organization remained poorly understood. We studied hemidesmosome…
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  • Science Lab Topic: Super-Resolution

    Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex

    Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively investigated, the…
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  • Science Lab Topic: Super-Resolution

    Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles

    Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been…
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  • Science Lab Topic: TIRF Microscopy

    Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

    Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…
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  • Science Lab Topic: TIRF Microscopy

    Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

    The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
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  • Widefield fluorescence image of brest carcinoma tumor cells expressing GFP tagged cell adhesion Molecule CD44 that is expressed on the cell membrane, imagined in TIRF.
    Science Lab Topic: TIRF Microscopy

    Total Internal Reflection Fluorescence (TIRF) Microscopy

    Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
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  • Science Lab Topic: TIRF Microscopy

    TIRF Microscopy Explores Cellular Transport Processes

    Due to their special role in organ function and the exchange of biological components some body cells developed certain polarization characteristics. These are reflected in differences of their plasma…
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  • Science Lab Topic: Live-Cell Imaging

    TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

    Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional…
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  • Science Lab Topic: Live-Cell Imaging

    Exploring Cell Logistics

    Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a…
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