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  • Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green).

    Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms

    Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics: Quantitative spectroscopic imaging of label-free or chemically tagged…
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  • How to prepare samples for Stimulated Raman Scattering

    Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows researchers to probe the endogenous chemistry or…
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  • Step by Step Guide to the Molecular Basics of CARS Microscopy

    CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial…
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  • Stimulated Raman Scattering Microscopy Probes Neurodegenerative Disease

    Despite decades of research, the molecular mechanisms underlying some of the most severe neurodegenerative diseases, such as Alzheimer’s or Parkinson’s, remain poorly understood. The progression of…
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  • Coherent Raman Scattering Microscopy Publication List

    CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…
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  • Coherent Raman Scattering (CRS) Microscopy: A vast and growing range of applications revealed

    Leica Microsystems‘ Workshop on Coherent Raman Scattering (CRS) Microscopy attracted 30 enthusiastic participants from all over the world to beautiful Konstanz, Germany! The group enjoyed a number of…
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  • Direct visualization of the arterial wall water permeability barrier using CARS microscopy

    The artery wall is equipped with a water permeation barrier that allows blood to flow at high pressure without significant water leak. The precise location of this barrier is unknown despite its…
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  • Chemical Basis for Alteration of an Intraocular Lens Using a Femtosecond Laser

    The chemical basis for the alteration of the refractive properties of an intraocular lens with a femtosecond laser was investigated. Three different microscope setups have been used for the study:…
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  • Coupling of vinculin to F-actin requires Syndecan-4 proteoglycan

    Heparan Sulfate Proteoglycans act as a cell surface co-receptors for many proteins in which a signal is sent downstream via its cytosolic domain. The role of syndecan-4 proteoglycans in FA assembly…
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  • Unmask the Hidden without Probes: CARS

    Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul…
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  • Detectors for Sensitive Detection: HyD

    This article discusses detectors (more precisely: sensors), that are employed in single point, i.e. true confocal scanning microscopes. The sensors in such systems are usually photomultiplier tubes.…
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  • CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules

    Coherent anti-Stokes Raman scattering (CARS) microscopy is a technique that generates images based on the vibrational signatures of molecules. This imaging methods does not require labeling, yet…
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  • An Introduction to CARS Microscopy

    CARS overcomes the drawbacks of conventional staining methods by the intrinsic characteristics of the method. CARS does not require labeling because it is highly specific to molecular compounds which…
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  • Good Vibrations

    In recent years, new molecular imaging techniques, such as coherent anti-Stokes Raman scattering microscopy (CARS), have been developed for rapid vibrational imaging of living cells.
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  • CARS and Confocal

    The most important drawback of single-photon and multiphoton confocal microscopy is the need to label the specimen. CARS (Coherent Anti-Stokes Raman Spectroscopy) addresses this issue because it is…
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