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光激活、光转化和光控开关荧光蛋白
荧光蛋白(FP)如GFP、YFP或DsRed都是可视化观察活细胞中细胞组分的强大工具。尽管如此,目前仍然会有传统FP达到极限的情形发生。普通FP无法观察特定兴趣蛋白中专有、空间有限的蛋白质群体,因为它们在整个细胞中都有表达。此时,光激活、光转化和光控开关荧光蛋白就登上了舞台。荧光套件中的成员可以从非荧光状态激活,可以改变发射光谱,甚至可以逆转式地“开启和关闭”。借助这些“光学荧光笔”,研究人员可以…
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Rodent and Small-Animal Surgery
Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.
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Investigating Fruit Flies (Drosophila melanogaster)
Learn how to image and investigate Drosophila fruit fly model organisms efficiently with a microscope for developmental biology applications from this article.
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Studying Caenorhabditis elegans (C. elegans)
Find out how you can image and study C. elegans roundworm model organisms efficiently with a microscope for developmental biology applications from this article.
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声光调制在全光谱型激光共聚焦显微镜系统的应用
荧光最显著的特征是照射光(激发光)和检测光(发射光)颜色之间的偏移,称为斯托克斯位移。因此,在荧光成像中,不仅要将激发光和发射光的相应波长过滤出来,还需要将激发光从发射光中分离。过去,通常用平面光学元件,包括灰色或彩色滤光片和反射镜进行滤光和分光。虽然有多种平面光学元件可供使用,但固定的规格和低切换效率使其在使用上具有局限性,并且采用不同角度或梯度的涂层作为激发光和发射光的调谐方法也被证实并不可行…
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视频教程: 如何调节荧光光源的灯泡位置
荧光激发的传统光源是带汞灯的荧光灯管。实现明亮均匀激发的前提条件是灯泡在灯罩内正确对中和对齐。
本视频教程介绍了一种简易的方法,用于调节荧光灯管中的汞灯的位置。
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Fluorescence Correlation Spectroscopy (FCS)
Fluorescence correlation spectroscopy (FCS) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as the diffusion time, number of molecules or dark…