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荧光寿命成像显微术

寿命成像:荧光不仅仅只有颜色信息

过去,寿命成像一直是一种缓慢、复杂的专业化技术。 只有经验丰富的显微镜专家或物理学家才会使用这种技术。 徕卡显微系统公司处于当今荧光寿命成像技术的前沿。 我们的系统使寿命成像比以往更快、更易于使用,将这项技术的各种优势引入日常的共聚焦成像实验中。
 

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通过荧光寿命信息改进共聚焦成像效果

荧光寿命成像显微术 (FLIM) 是一种利用荧光染料固有特性的成像技术。 除了具有特有的发射光谱外,每个荧光分子还有特有的寿命,它反映了荧光基团在发射光子之前处于激发态的时间。 除了标准的荧光强度测量外,寿命分析还可以提供其他信息。

Single slice taken from a zebrafish heart showing the ventricle with an injury in the lower area. Image shows the nuclei of all cells (blue), nuclei of the cardiomyocyte (heart muscle cells, green) and the proliferating cells (red). Image courtesy of Laura Peces-Barba Castaño, Max Planck Institute for Heart and Lung Research.

提高质量

荧光寿命成像提供了额外的信息,有助提高共聚焦成像的质量。 它非常适合用于区分荧光发射光谱重叠的荧光探针,或消除不需要的背景荧光信号。

功能成像

FLIM 是一种用于测量和量化成像数据的重要应用。 由于寿命信息与荧光基团浓度无关,因此它非常适合用于功能成像。 功能成像超越了传统的分子样本位置和浓度记录,通过该技术可以进一步研究分子功能、相互作用及其环境。

新颖的成像应用

此外,FLIM 还支持多种新的成像应用,例如使用生物传感器研究细胞的微环境或基于样本的固有荧光特性进行成像。请参考此 应用文档,详细了解 FLIM 及其在 SP8 FALCON 系统中的成像应用。

S设立寿命成像标准

STELLARIS 8是未来导向性系统,具有扩展光谱的WLL和专有的Power HyD系列探测器选项,不仅提供STELLARIS 5核心系统的所有优势,还有额外增添的功能。

徕卡显微系统公司的 FLIM 解决方案让您能够利用荧光寿命的强大特性,从共聚焦成像实验的每一个样本中提取新的信息维度。 使用 FALCON 研究细胞生理学和探索细胞动力学。 利用 STELLARIS 中的 Tausense 技术可以立即获得功能信息,例如代谢状态、酸碱度和离子浓度。

使用我们简化的集成平台,您可以在实验中获得新的视角,从而获得对实验问题的新解答。

快速和完全集成的终身成像技术

随着快速且完全整合的寿命成像技术 SP8 FALCON 的推出,徕卡显微系统公司为未来的寿命成像树立了标杆。 徕卡显微系统公司的 SP8 FALCON 系统荣获了著名的百大科技研发奖。 此奖项表彰了该解决方案使用的创新的寿命成像方法。

STELLARIS 8 FALCON

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