Loading...
Principles of Multiphoton Microscopy for Deep Tissue Imaging
This tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking advantage of the reduced scattering…
Loading...
表面计量学简介
本报告简要讨论了几种常用于评估表面形貌(也称为表面纹理或表面光洁度)的重要计量技术和标准定义。随着纳米技术、薄涂层以及电路和装置小型化的出现,表面计量学已成为一个极其重要的科学和工程领域。
Loading...
超薄切片介绍
对样本开展研究时,为了以纳米级分辨率显示其精细结构,通常会使用到电子显微镜。电子显微镜有两种类型:扫描电子显微镜(SEM)用于对样本表面成像,以及需要使用极薄电子透明样本的透射电子显微镜(TEM)。因此,使用电子显微镜对样本内部的精细结构进行成像时,此类技术解决方案需要制作出非常薄的样本切片。被称为超显微技术的样本制备方法可以产生具有最小伪影的超薄切片(厚度20-150nm)。在切片过程中,样本的…
Loading...
How To Create EDOF (Extended Depth of Focus) Images
Watch this video to see how you can rapidly record sharp optical microscope images of samples with a large height variation. This is done with the optional Extended Depth of Focus (EDOF) function of…
Loading...
Using Mounting Frames for Light Sheet Sample Preparation
Sample handling is an important topic in the context of Light Sheet Microscopy. The TCS SP8 DLS integrates Light Sheet technology into an inverted confocal platform and can hence make use of general…
Loading...
Using a Rotation Device for Light Sheet Sample Mounting
The TCS SP8 DLS from Leica Microsystems is an innovative concept to integrate the Light Sheet Microscopy technology into the confocal microscope. Due to its unique optical architecture samples can be…
Loading...
Consumables for Laser Microdissection
There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own…
Loading...
冷冻透射电子显微镜的投入式冷冻技术:基本原理
透射电子显微镜(TEM)所需的高真空度极大地减弱了研究自然存在于水相中的标本的能力:将“湿”标本暴露在远低于水蒸汽压的压力下,将导致水相在真空中迅速沸腾,对标本的结构造成破坏性后果。因此,在常规TEM中使用了各种方法在显微观察前干燥标本——这样一个样品制备步骤,通常导致实验结果出现假象。
Loading...
Video Tutorials: Filling and Assembling of Different Carriers for High-Pressure Freezing
High pressure freezing (HPF) is a cryo-fixation method primarily for biological samples, but also for a variety of non-biological materials. It is a technique that yields optimal preservation in many…