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系统评估透明化方案的质量标准
三维多细胞球体由于比标准二维细胞培养更能模拟体内肿瘤环境,因而在研究肿瘤行为及评估药理活性物质反应方面具有重要价值。然而,尽管球体具备生物学上的优势,使用现有的显微镜解决方案观察大型三维球体内部最深层的单个细胞依然面临挑战,主要是因为光散射导致的穿透问题。
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How FLIM Microscopy Helps to Detect Microplastic Pollution
The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…