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显微镜科学与教学知识中心

显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！
Application example of hyperspectral imaging

共聚焦多色成像在癌症研究和免疫学中的潜力

在本次网络研讨会上，来自莫纳什制药科学研究所的CameronNowell和他的同事将分享他们在多重成像方面的经验，以及他们通过巧妙的共聚焦成像采集和利用FLIM等其他多重成像模式所取得的成果。
[Translate to chinese:] In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD

神经血管病理学之窗

探索先天性免疫如何在神经血管病变后持续产生有害影响，以及能够对这些事件进行纵向研究的技术发展。
[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.

可重复性、协作和新成像技术的力量

在本次网络研讨会上，您将了解到影响显微镜可重复性的因素，有哪些资源和举措可用于改善显微镜教育并提高其严谨性和可重复性以及研究人员、成像科学家和显微镜供应商之间的合作如何推动创新和采用新技术。
[Translate to chinese:] Single-cell Huh-7D12 spheroid imaged using a Leica SP8 DLS microscope system. The spheroid was clarified using the Sucrose protocol.

系统评估透明化方案的质量标准

三维多细胞球体由于比标准二维细胞培养更能模拟体内肿瘤环境，因而在研究肿瘤行为及评估药理活性物质反应方面具有重要价值。然而，尽管球体具备生物学上的优势，使用现有的显微镜解决方案观察大型三维球体内部最深层的单个细胞依然面临挑战，主要是因为光散射导致的穿透问题。

How FLIM Microscopy Helps to Detect Microplastic Pollution

The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…

Development of Fluorescence Lifetime Imaging Microscopy (FLIM) and its Relevance for Functional Imaging

Prof. Ammasi Periasamy, Director, Keck Center for Cellular Imaging, University of Virginia, was interviewed by Dr. Giulia Ossato, Product Manager functional imaging, during Leica Microsystems Meets…

FRAP实验步骤式指南

漂白后荧光恢复（FRAP）已被认定为观察大分子平移扩散过程方面使用最为广泛的一种方法。由此产生的信息可用于确定动力学性质，如扩散系数、流动分数和荧光标记分子的传输速率。FRAP实验利用了短激光脉冲的荧光团辐照。先进的激光扫描显微镜如TCS…
Eukaryotic cells

Widefield Calcium Imaging with Calcium Indicator Fura2

In eukaryotic cells Ca2+ is one of the most widespread second messengers used in signal transduction pathways. Intracellular levels of Ca2+ are usually kept low, as Ca2+ often forms insoluble…
Sub-Femtolitre volume_Fluorescence correlation spectroscopy (FCS)

Fluorescence Correlation Spectroscopy (FCS)

Fluorescence correlation spectroscopy (FCS) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as the diffusion time, number of molecules or dark…
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