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A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
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FRAP实验步骤式指南
漂白后荧光恢复(FRAP)已被认定为观察大分子平移扩散过程方面使用最为广泛的一种方法。由此产生的信息可用于确定动力学性质,如扩散系数、流动分数和荧光标记分子的传输速率。FRAP实验利用了短激光脉冲的荧光团辐照。先进的激光扫描显微镜如TCS…
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Fluorescence Recovery after Photobleaching (FRAP) and its Offspring
FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…