联系我们
显微镜科学与教学知识中心

显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记,你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区,分享您的专业知识!
[Translate to chinese:] Elucidate cancer development on sub-cellular level by in-vivo like tumor spheroid models.

利用光片显微镜改进三维细胞生物学工作流程

了解癌症发生过程中的亚细胞机制对于癌症治疗至关重要。常见的细胞模型涉及作为单层生长的癌细胞。然而,这种方法忽视了肿瘤细胞与其周围微环境之间的三维相互作用。为了贴近自然环境理解恶性肿瘤的发展和进程,对癌症微环境的详细表征至关重要。

解析视场数(RFN)

光学显微镜的视场数(FN)表示视野大小(FOV)。它对应于中间图像中通过目镜可以观察到的区域。虽然我们不能一次观测到很大的视野,但人眼可以扫描并整合整个视野的结构特征。重要的是,该领域的大小和分辨率适合人眼能力。

什么是视场扫描仪?

视场扫描器是单点共聚焦显微镜中使用的振镜扫描组件,可对大视场尺寸进行正确的光学记录。与传统的双镜扫描仪相比,视场扫描仪采用了三镜概念,在不影响高速扫描的情况下提供了更高的照明均匀性。振镜扫描仪的速度和位置可控,允许缩放和平移功能以及调整扫描频率。它们还可以进行静止点照明,例如 FCS 测量所需的照明。

什么是串联扫描器?

串联扫描器集成了两种不同类型的扫描技术于一体,以实现真正的共焦点扫描。该系统包括一个三镜头扫描基座,其x轴扫描器可以与一个电动装置进行互换。这一组合不仅允许通过FOV扫描器实现大面积高分辨率扫描,还可以通过共振扫描器对极速过程进行扫描,两者均在同一仪器中完成。

See More Than Just Your Image

Despite the emergence of new imaging methods in recent years, true 3D resolution is still achieved by Confocal Laser Scanning Microscopy (CLSM). Through a combination of novel, extremely fast scanning…

Which Sensor is the Best for Confocal Imaging?

The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.

Primary Beam Splitting Devices for Confocal Microscopes

Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam…
HeLa cells stimulated with LPS. Image has been subjected to deconvolution.

Chronic Inflammation Under the Microscope

In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can…
C. elegans

Studying Caenorhabditis elegans (C. elegans)

Find out how you can image and study C. elegans roundworm model organisms efficiently with a microscope for developmental biology applications from this article.
Scroll to top