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How to Perform Dynamic Multicolor Time-Lapse Imaging
Live-cell imaging sheds light on diverse cellular events. As many of these events have fast dynamics, the microscope imaging system must be fast enough to record every detail. One major advantage of…
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Multiplexing through Spectral Separation of 11 Colors
Fluorescence microscopy is a fundamental tool for life science research that has evolved and matured together with the development of multicolor labeling strategies in cells tissues and model…
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低温光学显微镜的新成像工具
荧光显微镜图像能够为cryo-FIB加工提供定位支持,其质量决定了所制备薄片的结果。本文描述了LIGHTNING技术是如何显著提高图像质量,以及如何利用该技术基于荧光寿命的信息来辨别样品的不同结构。
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A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
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Find Relevant Specimen Details from Overviews
Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…
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精确三维定位,实现EM成像——掌握精髓
低温电子断层扫描(CryoET)是一种成像技术,可以让研究人员以亚纳米分辨率观察蛋白质和其他大生物分子。了解分子的形状和结构,包括口袋和裂隙,可以帮助研究人员设计能够像拼图一样附着于分子的药物。低温ET成像也因此成为了解和治疗疾病和失调的重要基础。
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The Potential of Coherent Raman Scattering Microscopy at a Glance
Coherent Raman scattering microscopy (CRS) is a powerful approach for label-free, chemically specific imaging. It is based on the characteristic intrinsic vibrational contrast of molecules in the…