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显微镜科学与教学知识中心

显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记,你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区,分享您的专业知识!

对囊泡运输的见解

STELLARIS 可为囊泡运输的动态、结构和机理研究提供完整的互补信息。
Living HeLa cells stained with WGA-488 (yellow), SPY-Actin (cyan), and SiR-Tubulin (magenta). Instant Computational Clearing (ICC) was applied.

How to Perform Dynamic Multicolor Time-Lapse Imaging

Live-cell imaging sheds light on diverse cellular events. As many of these events have fast dynamics, the microscope imaging system must be fast enough to record every detail. One major advantage of…
Spectral separation of 11 fluorophores coupled to polystyrene beads on a STELLARIS confocal system.

Multiplexing through Spectral Separation of 11 Colors

Fluorescence microscopy is a fundamental tool for life science research that has evolved and matured together with the development of multicolor labeling strategies in cells tissues and model…
[Translate to chinese:] Projection of a confocal z-stack. Sum159 cells, human breast cancer cells kindly provided by Ievgeniia Zagoriy, Mahamid Group, EMBL Heidelberg, Germany. Blue–Hoechst - indicates nuclei, Green–MitoTracker mitochondria, and red–Bodipy - lipid droplets

低温光学显微镜的新成像工具

荧光显微镜图像能够为cryo-FIB加工提供定位支持,其质量决定了所制备薄片的结果。本文描述了LIGHTNING技术是如何显著提高图像质量,以及如何利用该技术基于荧光寿命的信息来辨别样品的不同结构。
[Translate to chinese:] Transverse histological cut of a rabbit tongue. 50 Mpixels images (2326 µm x 1739 µm) in 14 x 18 tiles. Lifetime gives an additional contrast that allows to differentiate different structures in histological stainings.

A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
[Translate to chinese:] Zebrafish heart showing the ventricle with an injury in the lower area

斑马鱼心脏损伤后心肌细胞增殖现象

本期MicaCam聚焦于斑马鱼相关研究(斑马鱼)。不同于其他哺乳动物的心脏细胞,斑马鱼的心脏细胞能够在受损之后完全再生。
Identification of distinct structures_roundworm_Ascaris_female

Find Relevant Specimen Details from Overviews

Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…
[Translate to chinese:] LNG-non-LNGHeLa cells labeled with light blue –Hoechst, Nuclei

精确三维定位,实现EM成像——掌握精髓

低温电子断层扫描(CryoET)是一种成像技术,可以让研究人员以亚纳米分辨率观察蛋白质和其他大生物分子。了解分子的形状和结构,包括口袋和裂隙,可以帮助研究人员设计能够像拼图一样附着于分子的药物。低温ET成像也因此成为了解和治疗疾病和失调的重要基础。
Multi-color SRS image of a tri-cellular cancer spheroid

The Potential of Coherent Raman Scattering Microscopy at a Glance

Coherent Raman scattering microscopy (CRS) is a powerful approach for label-free, chemically specific imaging. It is based on the characteristic intrinsic vibrational contrast of molecules in the…
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