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显微镜科学与教学知识中心

显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记,你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区,分享您的专业知识!
Multicolor fixed STED image. Inner ear section, mouse, TauSTED Xtend 589 on AF488 and TauSTED Xtend 775 on AF633-Phalloidin. Sample courtesy of Dennis Derstrof, Klinik für Hals-, Nasen und Ohrenheilkunde, Universität Marburg & Prof. Dr. Dominik Oliver aus dem Institut für Physiologie und Pathophysiologie, Abteilung für Neurophysiologie, Universität Marburg.

Extended Live-cell Imaging at Nanoscale Resolution

Extended live-cell imaging with TauSTED Xtend. Combined spatial and lifetime information allow super-resolution microscopy at extremely low light dose.
An 8-color spectral unmixing result from a hyperspectral SRS (stimulated Raman scattering) dataset, showing the biochemically distinct structures of a fresh, untreated apple slice.

How to Prepare Samples for Stimulated Raman Scattering (SRS) imaging

Find here guidelines for how to prepare samples for stimulated Raman scattering (SRS), acquire images, analyze data, and develop suitable workflows. SRS spectroscopic imaging is also known as SRS…

Coherent Raman Scattering Microscopy Publication List

CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…
[Translate to chinese:] In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD

神经血管病理学之窗

探索先天性免疫如何在神经血管病变后持续产生有害影响,以及能够对这些事件进行纵向研究的技术发展。
[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.

可重复性、协作和新成像技术的力量

在本次网络研讨会上,您将了解到影响显微镜可重复性的因素,有哪些资源和举措可用于改善显微镜教育并提高其严谨性和可重复性以及研究人员、成像科学家和显微镜供应商之间的合作如何推动创新和采用新技术。
AI-based workflow for fast rare event detection in living biological samples using Autonomous Microscopy powered by Aivia

人工智能显微成像能够高效检测稀有事件

对稀有事件进行定位和选择性成像是许多生物样本研究过程的关键。然而,由于时间限制和高度的复杂性,有些实验无法做到,从而限制了获得新发现的前景。通过基于人工智能的显微成像检测稀有事件,这种工作流程将智能样本导航、图像采集工具和人工智能驱动的图像分析等不同功能融合起来共同协作,能够克服上述局限性。

Virtual Reality Showcase for STELLARIS Confocal Microscopy Platform

In this webinar, you will discover how to perform 10-color acquisition using a confocal microscope. The challenges of imaged-based approaches to identify skin immune cells. A new pipeline to assess…

免疫细胞在组织样品中的共聚焦成像

在本次网络研讨会中,您将探索如何使用共聚焦显微镜对组织样品进行10色成像,并了解这一技术如何有助于评估皮肤免疫状况。
[Translate to chinese:] Combining spectrally resolved detection and fluorescence lifetime multiplexing

使用有机荧光团的活细胞荧光寿命多标技术

点播网络研讨会: 如何利用荧光寿命多标技术结合光谱分辨检测技术对更多亚细胞目标进行成像。
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