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如何成功进行活细胞光电关联
Coral Life 提供了简化的活细胞 CLEM 解决方案，用于深入了解细胞成分随时间发生的结构变化。除了工作流程手册中描述的技术处理外，本文还提供了成功进行实验的其他知识。
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How to Successfully Implement Coral Life
The live-cell CLEM workflow allows you to capture dynamic information related to a relevant biological process as it happens and put these observations into their ultrastructural context. The Leica…
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Advancing Cellular Ultrastructure Research
Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the enteric glycocalyx. Freeze-fracture and etching are two…
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冷冻光电联用（Cryo-CLEM）之旅
本文主要介绍Cryo-CLEM技术及其为科学家带来的便益。此外，还特别说明了一些相关文献。
近期在冷冻电子显微镜工作流程领域取得的技术进步，让我们能够获取到细胞蛋白质社会学的3D数据，其分辨率更是达到前所未有的1纳米以下。工作流程中有一个步骤，需要从样品获取目标位置纳米级分辨率的图像，而要得到这样的结果，就需要用到冷冻光学显微镜。这种显微镜如果用于低温电子显微镜工作流程，通常就称为Cryo…
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![[Translate to chinese:] 3D reconstruction of an intercellular bridge in a C. elegans embryo](/fileadmin/_processed_/0/9/csm_cryo-workflow-lp-mz_d30fabea6b.jpg "[Translate to chinese:] 3D reconstruction of an intercellular bridge in a C. elegans embryo")
下载 EM 工作流程解决方案手册
这本 26 页的工作流程手册中讨论的解决方案已被证明能够提供快速、可靠和可重复的结果。如果您对自己的工作流程有特殊要求，或对此处显示的主题有任何疑问，我们的徕卡专家将随时乐意为您提供帮助。
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Exploring the Structure and Life Cycle of Viruses
The SARS-CoV-2 outbreak started in late December 2019 and has since reached a global pandemic, leading to a worldwide battle against COVID-19. The ever-evolving electron microscopy methods offer a…
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![[Translate to chinese:] Cryo FIB lamella - Overlay of SEM and confocal fluorescence image. Target structure in yeast cells (nuclear pore proteine Nup159-Atg8-split Venus, red) marked by an arrow. Scale bar: 5 µm. Alegretti et al., Nature 586, 796-800 (2020).](/fileadmin/_processed_/c/d/csm_Targeting_Nuclear_Pore_Complexes_teaser_16478dc18a.jpg "[Translate to chinese:] Cryo FIB lamella - Overlay of SEM and confocal fluorescence image")
使用冷冻共聚焦显微镜定位活性循环核孔复合物
本文介绍了如何利用冷冻光学显微镜，尤其是冷冻共焦显微镜来提高冷冻工作流程的可靠性。评估了EM网格和样品的质量，并分析了目标结构的分布。本文展示了如何将冷冻共焦3D数据投射到SEM图像上，将感兴趣结构可靠地保留在FIB切割的薄片内，以便在冷冻TEM中进行进一步研究。
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Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…
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Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…