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Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection
During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome.…
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A Brief History of Light Microscopy – From the Medieval Reading Stone to Super-Resolution
The history of microscopy begins in the Middle Ages. As far back as the 11th century, plano-convex lenses made of polished beryl were used in the Arab world as reading stones to magnify manuscripts.…
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From Light to Mind: Sensors and Measuring Techniques in Confocal Microscopy
This article outlines the most important sensors used in confocal microscopy. By confocal microscopy, we mean "True Confocal Scanning", i.e. the technique that illuminates and measures one single…
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Video: The White Light Laser – How to Effectively Excite Multiple Fluorophores with a Single Light Source
The Leica White Light Laser produces a continuous spectral output between the wavelengths of 470 and 670 nm. It allows you to select 8 excitation lines from 3 trillion unique combinations for…
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Joseph Gall的视频讲座:显微镜的早期历史
Joseph Gall带我们了解早期显微镜的历史和细胞的发现。复式显微镜是在17世纪与望远镜一起发明的;然而,由于光学畸变,这些显微镜直到19世纪末才被广泛使用。在此期间,简单的显微镜在整个18世纪和19世纪被用于生物学的重大发现,包括对细胞核、纤毛、细胞、细菌和原生动物的首次描述。光学技术在19世纪中后期得到改善厚,复合显微镜就被用来发现染色体、有丝分裂和其他细胞结构。
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Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis
Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or…
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关于数码显微镜,您一直想知道但从未问过的问题
数码显微镜是显微镜领域的热门词汇之一,其中有几个事实值得了解。徕卡显微系统公司的产品经理 Georg Schlaffer 经常被客户和同事问及有关数码显微系统的问题。他与科学撰稿人 Jim DeRose 合作,为最重要的问题提供了全面的答案。
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Confocal and Light Sheet Imaging
Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the…
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Consumables for Laser Microdissection
There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own…