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显微镜科学与教学知识中心

显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记,你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区,分享您的专业知识!
[Translate to chinese:] Water Flea Daphnia imaged by Electron Microscopy. Courtesy of Mag. Dr. Gruber, University of Vienna, Austria

简化生物应用的电镜样品制备工作流程

电子显微镜(EM)一直处于超微分辨率生物成像的最前沿,它简化了生物应用的 电镜 样品制备工作流程。
[Translate to chinese:] Co-detection of 10 extracellular matrix proteins and 3 topographical tissue landmarks by multiplex immunostaining within a single high-grade fibrous hotspot from a human hepatocellular carcinoma

肝细胞癌中癌症干细胞位点的原位鉴定

在这里,我们探索了一种突破性的多重免疫检测方法,通过多重成像对细胞外基质(ECM)特征进行原位定位,从而识别肝细胞癌(HCC)内的癌症干细胞龛。
Image of a Siemens star, where the diameter of the 1st black line circle is 10 mm and the 2nd is 20 mm, taken via an eyepiece of a M205 A stereo microscope. The rectangles represent the field of view (FOV) of a Leica digital camera when installed with various C-mounts (red 0.32x, blue 0.5x, green 0.63x).

30000:1放大率到底意味着什么?

关于光学显微镜性能的一个重要标准是放大率。本报告将为数字显微镜用户提供有用的指南,以确定放大率值的有用范围。
Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light.

Epi-Illumination Fluorescence and Reflection-Contrast Microscopy

This article discusses the development of epi-illumination and reflection contrast for fluorescence microscopy concerning life-science applications. Much was done by the Ploem research group…
Esophageal tissue with a squamous cell carcinoma labelled with the 4 biomarkers PanCk, DAPI, NaKATPase, and Vimentin.

Discover how Multiplexed Bioimaging can Advance Cancer Research

Explore multiplexing with up to 60 biomarkers, enabling advanced tumor imaging approaches to gather precise, spatially-resolved single-cell data that helps enhance cancer research and clinical…
Prof. Nikolaos Bechrakis uses the Proveo 8 ceiling mounted microscope with EnFocus intraoperative OCT. Images provided by Prof. Nikolaos Bechrakis.

Posterior Segment Surgery: Benefits of Utilizing Intraoperative OCT

Learn about the value of intraoperative optical coherence tomography in posterior segment surgery to precisely locate, evaluate and manage pathologies.
[Translate to chinese:] Material sample with a large height, size, and weight being observed with an inverted microscope.

工业应用中倒置显微镜相较于正置显微镜的五大优势

使用倒置显微镜时,您需要从下方观察样本,因为倒置显微镜的光学元件位于样本下方,而使用正置显微镜时,您需要从上方观察样本。一直以来,倒置显微镜主要用于生命科学研究,因为重力将样本沉入含有水性溶液的托座底部,从上方则无法观察到太多内容。但近段时间以来,倒置显微镜在工业应用中也变得越来越流行。我们现在一起来了解倒置显微镜在工业应用中的优势。
[Translate to chinese:] Application example of hyperspectral imaging

多重共焦成像在癌症研究和免疫学中的潜力

在本次网络研讨会上,来自莫纳什制药科学研究所的 Cameron Nowell 和他的同事将分享他们在多重成像方面的经验,以及他们通过巧妙的共聚焦成像采集和利用 FLIM 等其他多重成像模式所取得的成果。
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