Justus Liebig University Gießen, Department of Agricultural Sciences, Nutritional Sciences and Environmental Management, Gießen, Germany
The Justus Liebig University is one of Germany’s top research universities featuring an extraordinarily broad range of subjects. Both rich in tradition and highly innovative, JLU is host to a number of projects which are beacons of German research.
In 1999 the former Faculty of Agriculture and Environmental Protection and the Faculty of Nutritional Sciences were verified to the new Faculty of Agricultural Sciences, Nutritional Sciences and Environmental Management. This provided the basis for a scientific view of the whole food chain - starting from primary production and effects on the environment via processing, quality control and distribution to the food consumer.
http://www.uni-giessen.de/cms/faculties/f09/view?set_language=en
激光显微切割技术导论
组织学和生物学样本的异质性通常要求在分子生物学分析前从周围组织中分离出特定的单个细胞或细胞群。激光显微切割（LMD）是一种高效选择性收集用于制备DNA、RNA、蛋白质或其他生物材料样本以供分析的方法。这是一种显微镜控制的操纵技术，利用聚焦激光束精确分离样本、细胞和组织。本文阐述了LMD的基本原理。
不同组织样品制备方法的RNA质量
本文介绍了样品制备过程和紫外激光显微切片（UV LMD）对小鼠脑组织冷冻切片RNA质量的影响。为在提取RNA时获取良好的结果，从高品质组织开始并在处理前后检查RNA质量至关重要。RNA完整性指数（RIN）以1到10的等级标准来显示样品质量。简言之，RIN数值越高，RNA质量越高。这项研究结果表明，可以利用紫外激光显微切片技术，可以从单个组织细胞中获取品质稳定的RNA。
