Bascom Palmer Eye Institute, FL, USA

Bascom Palmer’s multidisciplinary research program covers a broad spectrum, from the most basic investigation into the nature of molecules, cells and tissues, to the development of devices for clinical and surgical applications. Current areas of clinical research include age-related macular degeneration, glaucoma, diabetic retinopathy, diseases of the retina and optic nerve, ocular infections and cancers of the eye.

About Our Research | Bascom Palmer Eye Institute | University of Miami Health System

基于激光的视神经再生研究新方法

由于哺乳动物中枢神经系统(CNS)的自我修复能力有限以及传统损伤模型的不一致性,视神经再生是神经生物学的一大挑战。相比之下,爪蟾蝌蚪的视神经在受伤后可以再生,因此是研究轴突再生的分子和细胞机制的理想模型。在本应用说明中,我们展示了如何利用激光显微切割技术(LMD)对蝌蚪的视神经进行精确、一致的横切,从而开发出适合成像、转录组分析和功能恢复研究的高重复性损伤模型。
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