Tamara Straube , PhD

Tamara Straube studied Human Biology at the Philipps-University Marburg and graduated in 2009. During her PhD thesis at the Department of Cytobiology and Cytopathology in Marburg she worked on protein trafficking in polarized epithelial cells and human kidney cancer and gained experience in TIRF, confocal and GSDIM microscopy. Afterwards she coordinated the Bioimaging Facility of the SFB593 at the Philipps-University Marburg. Since July 2013 she has employed by Leica Microsystems as an Application Manager for the Leica SR GSD.

Microscope image of cultured cells at the bottom of a dish.

如何对细胞培养进行快速正确的检测

本文介绍了对培养贴壁细胞系进行传代的一般工作流程及步骤说明。哺乳类细胞体外培养是在癌症、药物开发、组织工程、干细胞、疾病细胞和分子生物学等生物医学研究领域进行临床和药物研究的重要模型。要想成功维持细胞系，需要通过控制生长条件来维持细胞生理和表型的稳定性。定期监测细胞生长，对细胞进行传代培养以确保连续性。

超分辨率 GSDIM 显微镜

纳米级技术GSDIM（基态耗尽显微镜后单分子返回）提供了细胞内蛋白质和其他生物分子空间排列的详细图像。目前市场上已有首个商业系统（Leica SR GSD），它正在帮助将GSDIM技术推广给更多研究实验室和成像中心的用户。