Chloe Moulin

Chloe Moulin is a graduate of the University of Vermont and a PhD candidate in Molecular Cell and Developmental Biology at the University of Miami, Bascom Palmer Eye Institute. Her doctoral research leverages the Xenopus laevis model to explore the cellular and molecular mechanisms of optic nerve regeneration after traumatic injury. She combines high‑resolution confocal microscopy, live-imaging of retinal neurons, and advanced molecular approaches to compare regenerative dynamics in mammalian and amphibian species with spatial and temporal detail. Her work includes 3D-imaging to map axonal regrowth in-vivo and functional calcium imaging to track light‑evoked and injury‑induced signaling along the visual pathway. Through this work, she aims to identify targetable pathways for vision‑preserving therapies, with the ultimate goal of advancing regenerative medicine treatments for vision restoration.

Optic nerve regeneration in the Xenopus laevis tadpole.

基于激光的视神经再生研究新方法

由于哺乳动物中枢神经系统（CNS）的自我修复能力有限以及传统损伤模型的不一致性，视神经再生是神经生物学的一大挑战。相比之下，爪蟾蝌蚪的视神经在受伤后可以再生，因此是研究轴突再生的分子和细胞机制的理想模型。在本应用说明中，我们展示了如何利用激光显微切割技术（LMD）对蝌蚪的视神经进行精确、一致的横切，从而开发出适合成像、转录组分析和功能恢复研究的高重复性损伤模型。